IRIS
PELICCI, SIMONE
 Distribuzione geografica
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Continente #
EU - Europa 1.049
Totale 1.049
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Nazione #
IT - Italia 1.049
Totale 1.049
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Città #
Genova 695
Rapallo 166
Genoa 154
Vado Ligure 29
Bordighera 5
Totale 1.049
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Nome #
A Liquid Tunable Microscope as a New Paradigm in Optical Microscopy to Paint 4D Chromatin Organisation in the Cell Nucleus 97
SPLIT-STED Imaging of Nuclear Structures 87
FLIM-FRET of Chromatin in Live Cells using Two DNA-Binding Dyes 82
NANOSCALE INVESTIGATION OF NUCLEAR STRUCTURES BY TIME-RESOLVED MICROSCOPY 82
Chromatin Nanoscale Organization Investigated by FLIM-FRET and STED Superresolution Microscopy 81
Chromatin Alterations in a Model of Oncogene Activation Studied by Advanced Fluorescence Microscopy 81
A Novel Viewpoint to Analyze Structured Illumination Microscopy (Sim) Data 79
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus 78
Probing Chromatin Organization by Sorting of Short Sequence Fluorescence Correlation Spectroscopy 71
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM 70
Intensity Sorted Fluorescence Correlation Spectroscopy: A Novel Method to Probe Nuclear Dynamics and Chromatin Organization in Living Cells 69
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells. 66
Improving SPLIT-STED super-resolution imaging with tunable depletion and excitation power 42
Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes 31
Structured Illumination Microscopy as a Tool to Investigate Oncogene-Induced Alterations in Chromatin Organization 28
Nanoscale Distribution of Nuclear Sites Analyzed by Superresolution STED Image Cross-Correlation Spectroscopy 28
Totale 1.072
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Categoria #
all - tutte 4.925
article - articoli 2.605
book - libri 0
conference - conferenze 2.095
curatela - curatele 0
other - altro 0
patent - brevetti 0
selected - selezionate 0
volume - volumi 0
Totale 9.625
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Totale Lug Ago Sett Ott Nov Dic Gen Feb Mar Apr Mag Giu
2019/2020302 0 0 21 24 26 37 52 24 29 50 37 2
2020/2021125 17 4 6 26 4 6 6 15 9 15 9 8
2021/2022199 11 5 5 31 3 28 8 39 23 22 5 19
2022/2023202 23 24 7 11 25 30 0 14 38 0 29 1
2023/2024117 3 17 0 16 12 18 10 8 6 9 6 12
2024/202535 10 25 0 0 0 0 0 0 0 0 0 0
Totale 1.072