PELICCI, SIMONE

PELICCI, SIMONE  

100020 - Dipartimento di Fisica  

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Chromatin Alterations in a Model of Oncogene Activation Studied by Advanced Fluorescence Microscopy 1-gen-2019 Lanzano′, Luca; Oneto, Michele; Cainero, Isotta; Pelicci, Simone; Sarmento, Maria; Scipioni, Lorenzo; Faretta, Mario; Furia, Laura; Ivan Dellino, Gaetano; Giuseppe Pelicci, Pier; Bianchini, Paolo; Diaspro, Alberto
Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes 1-gen-2019 Pelicci, S.; Diaspro, A.; Lanzano, L.
Chromatin Nanoscale Organization Investigated by FLIM-FRET and STED Superresolution Microscopy 1-gen-2019 Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Cainero, Isotta; Barboro, Paola; Diaspro, Alberto; Lanzano′, Luca
FLIM-FRET of Chromatin in Live Cells using Two DNA-Binding Dyes 1-gen-2018 Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Diaspro, Alberto; Lanzanò, Luca
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells. 1-gen-2018 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
Improving SPLIT-STED super-resolution imaging with tunable depletion and excitation power 1-gen-2020 Pelicci, S.; Tortarolo, G.; Vicidomini, G.; Diaspro, A.; Lanzano, L.
Intensity Sorted Fluorescence Correlation Spectroscopy: A Novel Method to Probe Nuclear Dynamics and Chromatin Organization in Living Cells 1-gen-2019 Di Bona, Melody; Pelicci, Simone; Cainero, Isotta; Vicidomini, Giuseppe; Mazza, Davide; Mancini, Michael A.; Diaspro, Alberto; Lanzano', Luca
A Liquid Tunable Microscope as a New Paradigm in Optical Microscopy to Paint 4D Chromatin Organisation in the Cell Nucleus 1-gen-2018 Diaspro, Alberto; Cainero, Isotta; Lanzanò, Luca; Bianchini, Paolo; Vicidomini, Giuseppe; Cella Zanacchi, Francesca; Pesce, Luca; Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Faretta, Mario; Barboro, Paola; Le Gratiet, Aymeric
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus 1-gen-2018 Diaspro, Alberto; Anthony, Nicholas; Bianchini, Paolo; Cainero, Isotta; Di Bona, Melody; Lanzanò, Luca; Le Gratiet, Aymeric; Marongiu, Riccardo; Oneto, Michele; Pelicci, Simone; Pesce, Luca
Nanoscale Distribution of Nuclear Sites Analyzed by Superresolution STED Image Cross-Correlation Spectroscopy 1-gen-2020 Oneto, M; Scipioni, L; Sarmento, M; Cainero, I; Cerutti, E; Pelicci, S; Furia, L; Pelicci, Pg; Dellino, Gi; Bianchini, P; Faretta, M; Gratton, E; Diaspro, A; Lanzano, L
NANOSCALE INVESTIGATION OF NUCLEAR STRUCTURES BY TIME-RESOLVED MICROSCOPY 26-mar-2020 Pelicci, Simone
A Novel Viewpoint to Analyze Structured Illumination Microscopy (Sim) Data 1-gen-2019 Cainero, Isotta; Pelicci, Simone; Di Bona, Melody; Diaspro, Alberto; Lanzano', Luca
Probing Chromatin Organization by Sorting of Short Sequence Fluorescence Correlation Spectroscopy 1-gen-2018 Di Bona, Melody; Pelicci, Simone; Vicidomini, Giuseppe; Cammarota, Eugenia; Mazza, Davide; Diaspro, Alberto; Lanzanò, Luca
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM 1-gen-2019 Castello, M.; Tortarolo, G.; Buttafava, M.; Deguchi, T.; Villa, F.; Koho, S.; Pesce, L.; Oneto, M.; Pelicci, S.; Lanzano, L.; Bianchini, P.; Sheppard, C. J. R.; Diaspro, A.; Tosi, A.; Vicidomini, G.
SPLIT-STED Imaging of Nuclear Structures 1-gen-2018 Lanzano', Luca; Sarmento, Maria J.; Scipioni, Lorenzo; Oneto, Michele; Pelicci, Simone; Di Bona, Melody; Pesce, Luca; Faretta, Mario; Furia, Laura; Dellino, Gaetano I.; Pelicci, Pier G.; Bianchini, Paolo; Diaspro, Alberto
Structured Illumination Microscopy as a Tool to Investigate Oncogene-Induced Alterations in Chromatin Organization 1-gen-2020 Cainero, Isotta; Cerutti, Elena; Pelicci, Simone; Faretta, Mario; Ivan Dellino, Gaetano; Giuseppe Pelicci, Pier; Diaspro, Alberto; Lanzano, Luca