PELICCI, SIMONE
PELICCI, SIMONE
100020 - Dipartimento di Fisica
A Liquid Tunable Microscope as a New Paradigm in Optical Microscopy to Paint 4D Chromatin Organisation in the Cell Nucleus
2018-01-01 Diaspro, Alberto; Cainero, Isotta; Lanzanò, Luca; Bianchini, Paolo; Vicidomini, Giuseppe; Cella Zanacchi, Francesca; Pesce, Luca; Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Faretta, Mario; Barboro, Paola; Le Gratiet, Aymeric
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
2019-01-01 Castello, M.; Tortarolo, G.; Buttafava, M.; Deguchi, T.; Villa, F.; Koho, S.; Pesce, L.; Oneto, M.; Pelicci, S.; Lanzano, L.; Bianchini, P.; Sheppard, C. J. R.; Diaspro, A.; Tosi, A.; Vicidomini, G.
Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes
2019-01-01 Pelicci, S.; Diaspro, A.; Lanzano, L.
FLIM-FRET of Chromatin in Live Cells using Two DNA-Binding Dyes
2018-01-01 Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Diaspro, Alberto; Lanzanò, Luca
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells.
2018-01-01 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
Improving SPLIT-STED super-resolution imaging with tunable depletion and excitation power
2020-01-01 Pelicci, S.; Tortarolo, G.; Vicidomini, G.; Diaspro, A.; Lanzano, L.
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus
2018-01-01 Diaspro, Alberto; Anthony, Nicholas; Bianchini, Paolo; Cainero, Isotta; Di Bona, Melody; Lanzanò, Luca; Le Gratiet, Aymeric; Marongiu, Riccardo; Oneto, Michele; Pelicci, Simone; Pesce, Luca
Probing Chromatin Organization by Sorting of Short Sequence Fluorescence Correlation Spectroscopy
2018-01-01 Di Bona, Melody; Pelicci, Simone; Vicidomini, Giuseppe; Cammarota, Eugenia; Mazza, Davide; Diaspro, Alberto; Lanzanò, Luca