The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated emission depletion (STED), excited fluorophores located on the periphery of the Point Spread Function (PSF) are completely switched off by a doughnut-shaped beam, producing an effective PSF of smaller size.
SPLIT-STED Imaging of Nuclear Structures
Pelicci, Simone;Di Bona, Melody;Pesce, Luca;Bianchini, Paolo;Diaspro, Alberto
2018
Abstract
The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated emission depletion (STED), excited fluorophores located on the periphery of the Point Spread Function (PSF) are completely switched off by a doughnut-shaped beam, producing an effective PSF of smaller size.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
