P0 is a myelin glycoprotein of the peripheral nervous system (PNS). It can bind as many as 6 different glycans, all at its one and unique glycosylation site, Asn93.This is in contrast to other myelin glycoproteins which have also one glycosylation site: PMP22 at Asn36, MOG at Asn31, but can bind only one glycan type containing usually HNK-1 epitope. The MAG has 10 glycosylation sites, and glycoprotein OMpg has 11 glycosylation sites, some of them contain HNK-1 epitope. The HNK-1 epitope is involved in cell interactions that controls cell type-specific neurite outgrowth, regeneration, myelin stability and is target for autoimmune IgM antibodies in human demyelinating neuropathies of the PNS. Beside myelin P0 glycoprotein, no data are available on other myelin glycoproteins. In this paper we review and analyze all published data on the chemical structure of glycans containing HNK-1 epitopes or sialic acid of bovine P0, PMP22, MOG and MAG. For bovine P0, the MW of glycan is in the range of 1294.56(GP3) Da - 2279.94(GP5) Da. The pI of glycosylated P0 protein varies from pH 9.32-9.46. The most charged is a glycan MS2 containing 3 sulfate groups and one glucuronic acid; the less charged is glycan BA2. All glycans contain 1 fucose and 1 galactose. The most mannose rich are MS2 and GP4, each has 4 mannoses; OPPE1 contains 5 N-acetylglucosamines and 1 sulfated glucuronic acid, GP4 contains 1 sialic acid.

Glycans of myelin proteins.

GRANDIS, MARINA
2015-01-01

Abstract

P0 is a myelin glycoprotein of the peripheral nervous system (PNS). It can bind as many as 6 different glycans, all at its one and unique glycosylation site, Asn93.This is in contrast to other myelin glycoproteins which have also one glycosylation site: PMP22 at Asn36, MOG at Asn31, but can bind only one glycan type containing usually HNK-1 epitope. The MAG has 10 glycosylation sites, and glycoprotein OMpg has 11 glycosylation sites, some of them contain HNK-1 epitope. The HNK-1 epitope is involved in cell interactions that controls cell type-specific neurite outgrowth, regeneration, myelin stability and is target for autoimmune IgM antibodies in human demyelinating neuropathies of the PNS. Beside myelin P0 glycoprotein, no data are available on other myelin glycoproteins. In this paper we review and analyze all published data on the chemical structure of glycans containing HNK-1 epitopes or sialic acid of bovine P0, PMP22, MOG and MAG. For bovine P0, the MW of glycan is in the range of 1294.56(GP3) Da - 2279.94(GP5) Da. The pI of glycosylated P0 protein varies from pH 9.32-9.46. The most charged is a glycan MS2 containing 3 sulfate groups and one glucuronic acid; the less charged is glycan BA2. All glycans contain 1 fucose and 1 galactose. The most mannose rich are MS2 and GP4, each has 4 mannoses; OPPE1 contains 5 N-acetylglucosamines and 1 sulfated glucuronic acid, GP4 contains 1 sialic acid.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/792213
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