Introduction Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. Methods This paper describes the procedure to obtain a 5 cc saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. Results A mean of 2.5 × 106 cells is isolated for each procedure; 35 % (875 000) of these are CD34 +/ CD45-cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLA-ABC) and negative (CD33, CD39, CD102, CD106, CD146, HLA-DR) cell-associated surface antigens, characterizing them as ASCs. Conclusions This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.
Isolement des cellules souches mésenchymateuses autologues dérivées du tissu adipeux pour la réparation des pertes de substance osseuse
RAPOSIO, Edoardo;
2016-01-01
Abstract
Introduction Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. Methods This paper describes the procedure to obtain a 5 cc saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. Results A mean of 2.5 × 106 cells is isolated for each procedure; 35 % (875 000) of these are CD34 +/ CD45-cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLA-ABC) and negative (CD33, CD39, CD102, CD106, CD146, HLA-DR) cell-associated surface antigens, characterizing them as ASCs. Conclusions This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.
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