Human osteoblast-like cell lines were established in vitro from spongious bone fragments and analyzed by means of indirect immunoperoxidase technique for the expression of HLA class I (A, B, C) and class II (DR) antigens when cultured in absence or after culture in presence of human recombinant cytokines such as IL-1, IL-6, tumor necrosis factor-(TNF-a) and interferon-?(IFN-?). The results showed that: 1) osteoblast-like bone cells, studied after the third and the fifth in vitro passage, appeared positive for IILA class I antigens but did not express HLA-DR molecules. 2) Following in vitro culture in the presence of the above cytokins, TNF-a and IFN-? only were able both to upregulate HLA-class I expression and to induce DR antigens on osteoblast cells. The above data may have some importance for the understanding of immune mechanisms regulation bone resorption at the bone remodeling sites.

Espressione di antigeni HLA da parte di osteoblasti umani coltivati in vitro.

SANGUINETI, FRANCESCA;
1992

Abstract

Human osteoblast-like cell lines were established in vitro from spongious bone fragments and analyzed by means of indirect immunoperoxidase technique for the expression of HLA class I (A, B, C) and class II (DR) antigens when cultured in absence or after culture in presence of human recombinant cytokines such as IL-1, IL-6, tumor necrosis factor-(TNF-a) and interferon-?(IFN-?). The results showed that: 1) osteoblast-like bone cells, studied after the third and the fifth in vitro passage, appeared positive for IILA class I antigens but did not express HLA-DR molecules. 2) Following in vitro culture in the presence of the above cytokins, TNF-a and IFN-? only were able both to upregulate HLA-class I expression and to induce DR antigens on osteoblast cells. The above data may have some importance for the understanding of immune mechanisms regulation bone resorption at the bone remodeling sites.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/486526
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