The primary objective of this study was to valorize Vitex agnus-castus residues in terms of phenolic compounds with antioxidant activity. The effects of extraction time (30-360 min), solid to liquid ratio (0.1-0.3 gDryBiomass/mlSolvent), type of solvent (methanol or ethanol) and different tissue types (leave, roots and seeds) on total polyphenols, o-diphenols, total flavonoids and anthocyanins were evaluated. The highest total polyphenol (31.5 mgCaffeicAcidEquivalent/gDryBiomass) and o-diphenol (12.4 mgCaffeicAcidEquivalent/gDryBiomass) contents were obtained from methanolic extract of leaves after 180 min using a solid/liquid ratio of 0.1 gDryBiomass/mlSolvent, while total flavonoids, reached a maximum value of 19.4 mgCatechinEquivalent/gDryBiomass after 360 min under the same conditions. Roots of Vitex agnus-castus were found to be a good source of anthocyanins with the highest yield of 0.62 mgMiricetinEquivalent/gDryBiomass using ethanol as a solvent (180 min and 0.2 gDryBiomass/mlSolvent). The maximum antiradical power (178.5 µlextract/µgDPPH) was exhibited by the methanolic leave extract obtained after 360 min at solid/liquid ratio of 0.3 gDryBiomass/mlSolvent.
Extraction of phenolic compounds from Vitex agnus-castus L.
ALIAKBARIAN, BAHAR;CASAZZA, ALESSANDRO ALBERTO;CONVERTI, ATTILIO;PEREGO, PATRIZIA
2012
Abstract
The primary objective of this study was to valorize Vitex agnus-castus residues in terms of phenolic compounds with antioxidant activity. The effects of extraction time (30-360 min), solid to liquid ratio (0.1-0.3 gDryBiomass/mlSolvent), type of solvent (methanol or ethanol) and different tissue types (leave, roots and seeds) on total polyphenols, o-diphenols, total flavonoids and anthocyanins were evaluated. The highest total polyphenol (31.5 mgCaffeicAcidEquivalent/gDryBiomass) and o-diphenol (12.4 mgCaffeicAcidEquivalent/gDryBiomass) contents were obtained from methanolic extract of leaves after 180 min using a solid/liquid ratio of 0.1 gDryBiomass/mlSolvent, while total flavonoids, reached a maximum value of 19.4 mgCatechinEquivalent/gDryBiomass after 360 min under the same conditions. Roots of Vitex agnus-castus were found to be a good source of anthocyanins with the highest yield of 0.62 mgMiricetinEquivalent/gDryBiomass using ethanol as a solvent (180 min and 0.2 gDryBiomass/mlSolvent). The maximum antiradical power (178.5 µlextract/µgDPPH) was exhibited by the methanolic leave extract obtained after 360 min at solid/liquid ratio of 0.3 gDryBiomass/mlSolvent.
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