We have performed electrical measurements on bilayer lipid membranes (BLMs) in the presence of nisin. The membranes were formed in 0.1 M KCI solution following the technique of Montal-Mueller as well as that of Rudin-Mueller. Nisin was dissolved in water and introduced in small amount into one side (cis-side) of the solution bathing the membrane to final concentrations of 10(-7)-10(-5) M. By applying external voltages of different polarity, membrane conductance increases were observed. In Montal-Mueller membranes, channels with elementary conductance of about 145 pS were detected both for trans-negative and trans-positive potentials (50 mV). Channels with elementary conductance around 400 pS were observed only for trans-negative potentials. The log-log plot of macroscopic conductance versus nisin concentration was linear with a slope of (1.0 +/- 0.1), showing that one nisin molecule is involved in channel formation.
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|Titolo:||Channel-formation activity of the lantibiotic nisin on bilayer lipid membranes|
|Data di pubblicazione:||1996|
|Citazione:||Channel-formation activity of the lantibiotic nisin on bilayer lipid membranes / A. Palmeri;I.M. Pepe;R. Rolandi. - In: THIN SOLID FILMS. - ISSN 0040-6090. - STAMPA. - 284-285(1996), pp. 822-824.|
|Appare nelle tipologie:||01.01 - Articolo su rivista|