The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets were measured simply and reproducibly. A rapid alk. extn. allows complete recovery of the compds. concerned. The metabolic ATP and ADP in the cytosolic fraction, the amt. released upon thrombin stimulation, and the ADP bound to F-actin also were evaluated. Anal. was performed by reversed-phase, isocratic HPLC on a 5-μm Lichrosorb RP-18 column with UV detection at 254 nm.

Alkaline extraction and reverse-phase high-performance liquid chromatography of adenine and pyridine nucleotides in human platelets.

MAZZEI, MAURO;BALBI, ALESSANDRO
1987-01-01

Abstract

The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets were measured simply and reproducibly. A rapid alk. extn. allows complete recovery of the compds. concerned. The metabolic ATP and ADP in the cytosolic fraction, the amt. released upon thrombin stimulation, and the ADP bound to F-actin also were evaluated. Anal. was performed by reversed-phase, isocratic HPLC on a 5-μm Lichrosorb RP-18 column with UV detection at 254 nm.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/382093
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 36
  • ???jsp.display-item.citation.isi??? 34
social impact