Evaluation of effects on bone tissue of different osteotomy techniques.

The aim of this study is to evaluate and compare the response of bone tissue after osteotomy carried out with either rotating cutters or with piezoelectric terminals.Bioptic samples of bone tissue were taken during operations with rotating burs and piezoelectric terminals to increase bone volume before implantology. Samples first underwent histomorphometric analysis. Subsequently, osteoblastic cells, obtained from different samples, were placed in culture and allowed to proliferate to in vitro evaluate the time to initiate growth and to reach confluence. Finally, a molecular biologic study by reverse transcription polymerase chain reaction was performed to evaluate the expression of typical osteoblastic molecular markers, such as osteoprotegerin and osteopontin.Histomorphometric analysis showed that the width of necrotic line on the osteotomic margins from samples taken using different techniques did not vary significantly. Moreover, the times of initial growth and of confluence in cells from the 2 groups did not show any statistically significant differences. However, a highly significant correlation was revealed between the age of the patient and the initial growth time and the confluence. Similarly, reverse transcription polymerase chain reaction showed that the osteoprotegerin and osteopontin expression levels did not change significantly according to the surgical technique used.In conclusion, osteotomies carried out with either instrument do not seem to substantially influence the vitality of the bone tissue. The variability of the expression levels of typical osteoblastic markers seems to be linked more to other factors than to the surgical technique used.