An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type 1 quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of => 30000, with sensitivity of 46.4% at a viral load of <30000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.
EVALUATION OF AN ULTRASENSITIVE P24 ANTIGEN ASSAY AS A POTENTIAL ALTERNATIVE TO HIV-1 RNA VIRAL LOAD IN RESOURCE-LIMITED SETTINGS
VARNIER, OLIVIERO;
2005-01-01
Abstract
An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type 1 quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of => 30000, with sensitivity of 46.4% at a viral load of <30000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.
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