Dihydrouracil (UH2)IUracil (U) ratio in plasma was determinated as a surrogate marker for dihydropyrimidine dehydrogenase (DPD) activity. The purpose of the study was to investigate the relationship between the UH2IU plasma ratio and the variation of DPD gene (DPYD), associated with a deficiency of DPD activity, in a patient who developed a severe adverse reaction to 5-fluorouracil. Patients'plasma sample and those of 20 healthy volunteers, used as control, were analyzed. The plasma concentrations of UH2 and U were assayed by HPLC-UV. UH2IU plasma ratio of the patient was 4.31; the mean ± SD ofUH2IU plasma ratios in controls was 5.26 ± 2.08. The UH2IU ratio of the patient was lower than the mean values recorded from a reference population suggesting a reduction of DPD activity according to haplotype of the patient, previously identified.
A case of 5-fluorouracil toxicity
Fucile C.;Marini V.;Robbiano L.;Mattioli F.;Martelli A.
2011-01-01
Abstract
Dihydrouracil (UH2)IUracil (U) ratio in plasma was determinated as a surrogate marker for dihydropyrimidine dehydrogenase (DPD) activity. The purpose of the study was to investigate the relationship between the UH2IU plasma ratio and the variation of DPD gene (DPYD), associated with a deficiency of DPD activity, in a patient who developed a severe adverse reaction to 5-fluorouracil. Patients'plasma sample and those of 20 healthy volunteers, used as control, were analyzed. The plasma concentrations of UH2 and U were assayed by HPLC-UV. UH2IU plasma ratio of the patient was 4.31; the mean ± SD ofUH2IU plasma ratios in controls was 5.26 ± 2.08. The UH2IU ratio of the patient was lower than the mean values recorded from a reference population suggesting a reduction of DPD activity according to haplotype of the patient, previously identified.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
