Genome sequence of hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and comparative genomics with V. cholerae

Grim, C. J.; Hasan, N. A.; Taviani, E.; Haley, B.; Chun, J.; Brettin, T. S.; Bruce, D. C.; Detter, J. C.; Han, C. S.; Chertkov, O.; Challacombe, J.; Huq, A.; Nair, G. B.; Colwell, R. R.

doi:10.1128/JB.00040-10

The genomes of Vibrio cholerae O1 Matlab variant MJ-1236, Mozambique O1 El Tor variant B33, and altered O1 El Tor CIRS101 were sequenced. All three strains were found to belong to the phylocore group 1 clade of V. cholerae, which includes the 7th-pandemic O1 El Tor and serogroup O139 isolates, despite displaying certain characteristics of the classical biotype. All three strains were found to harbor a hybrid variant of CTXΦ and an integrative conjugative element (ICE), leading to their establishment as successful clinical clones and the displacement of prototypical O1 El Tor. The absence of strain- and group-specific genomic islands, some of which appear to be prophages and phage-like elements, seems to be the most likely factor in the recent establishment of dominance of V. cholerae CIRS101 over the other two hybrid strains. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Genome sequence of hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and comparative genomics with V. cholerae

Grim C. J.;Hasan N. A.;Taviani E.;Haley B.;Chun J.;Brettin T. S.;Bruce D. C.;Detter J. C.;Han C. S.;Chertkov O.;Challacombe J.;Huq A.;Nair G. B.;Colwell R. R.

2010-01-01

Abstract

The genomes of Vibrio cholerae O1 Matlab variant MJ-1236, Mozambique O1 El Tor variant B33, and altered O1 El Tor CIRS101 were sequenced. All three strains were found to belong to the phylocore group 1 clade of V. cholerae, which includes the 7th-pandemic O1 El Tor and serogroup O139 isolates, despite displaying certain characteristics of the classical biotype. All three strains were found to harbor a hybrid variant of CTXΦ and an integrative conjugative element (ICE), leading to their establishment as successful clinical clones and the displacement of prototypical O1 El Tor. The absence of strain- and group-specific genomic islands, some of which appear to be prophages and phage-like elements, seems to be the most likely factor in the recent establishment of dominance of V. cholerae CIRS101 over the other two hybrid strains. Copyright © 2010, American Society for Microbiology. All Rights Reserved.