Purpose: Photobiomodulation of cells using near-infrared (NIR) monochromatic light can affect cell functions such as proliferation, viability, and metabolism in a range of cell types. Evidence for the effects of near-infrared light on endothelial cells has been reported, but the studies were mainly performed using VIS light emitted by low energy lasers, because NIR wavelengths seemed negatively stimulate these cells. Methods: Cell viability, free radical-induced oxidative stress, NF-κB activation, nitric oxide release, mitochondrial respiration and wound healing repair were assessed in human endothelial cells (HECV) irradiated with 808-nm diode laser light (laser set-up= 1W/cm2, 60 sec, 60J/cm2, CW vs measured parameter= 0.95W/cm2, 60 sec, 57J/ cm2, mode CW) emitted by an hand-piece with flat-top profile. Results: No difference in viability was detected between controls and HECV cells irradiated with 808-nm diode laser light for 60 s. Irradiated cells demonstrated higher proliferation rate and increased migration ability associated to moderate increase in ROS production without a significant increase in oxidative stress and oxidative-stress activated processes. Near-infrared light stimulated mitochondrial oxygen consumption and ATP synthesis in HECV cells. Short near-infrared irradiation did not affect viability of HECV cells, rather led to a stimulation of wound healing rate, likely sustained by ROS-mediated stimulation of mitochondrial activity. Conclusion: Our results demonstrating that near-infrared led to a shift from anaerobic to aerobic metabolism provide new insight into the possible molecular mechanisms by which photobiomodulation with 808-nm diode laser light protects against inflammation-induced endothelial dysfunction, seemingly promising to enhance their therapeutic properties

Photobiomodulation with 808-nm diode laser light promotes wound healing of human endothelial cells through increased reactive oxygen species production stimulating mitochondrial oxidative phosphorylation

Andrea Amaroli;Silvia Ravera;Francesca Baldini;Stefano Benedicenti;Isabella Panfoli;Laura Vergani
2019

Abstract

Purpose: Photobiomodulation of cells using near-infrared (NIR) monochromatic light can affect cell functions such as proliferation, viability, and metabolism in a range of cell types. Evidence for the effects of near-infrared light on endothelial cells has been reported, but the studies were mainly performed using VIS light emitted by low energy lasers, because NIR wavelengths seemed negatively stimulate these cells. Methods: Cell viability, free radical-induced oxidative stress, NF-κB activation, nitric oxide release, mitochondrial respiration and wound healing repair were assessed in human endothelial cells (HECV) irradiated with 808-nm diode laser light (laser set-up= 1W/cm2, 60 sec, 60J/cm2, CW vs measured parameter= 0.95W/cm2, 60 sec, 57J/ cm2, mode CW) emitted by an hand-piece with flat-top profile. Results: No difference in viability was detected between controls and HECV cells irradiated with 808-nm diode laser light for 60 s. Irradiated cells demonstrated higher proliferation rate and increased migration ability associated to moderate increase in ROS production without a significant increase in oxidative stress and oxidative-stress activated processes. Near-infrared light stimulated mitochondrial oxygen consumption and ATP synthesis in HECV cells. Short near-infrared irradiation did not affect viability of HECV cells, rather led to a stimulation of wound healing rate, likely sustained by ROS-mediated stimulation of mitochondrial activity. Conclusion: Our results demonstrating that near-infrared led to a shift from anaerobic to aerobic metabolism provide new insight into the possible molecular mechanisms by which photobiomodulation with 808-nm diode laser light protects against inflammation-induced endothelial dysfunction, seemingly promising to enhance their therapeutic properties
File in questo prodotto:
File Dimensione Formato  
LMS 2019_Amaroli et al.pdf

accesso chiuso

Tipologia: Documento in versione editoriale
Dimensione 1.37 MB
Formato Adobe PDF
1.37 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
11567_920725VerganiAM.pdf

accesso aperto

Descrizione: Accepted manuscript
Tipologia: Documento in Pre-print
Dimensione 1.44 MB
Formato Adobe PDF
1.44 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/920725
Citazioni
  • ???jsp.display-item.citation.pmc??? 18
  • Scopus 43
  • ???jsp.display-item.citation.isi??? 41
social impact