The present research investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine 5mg mL-1 both in the biofilm inhibition (BI 50%) and in the biofilm disaggregation tests (BD 20%). Considering A. naeslundi, BI values close to 80% were registered for oxalic acid at 1mg mL-1 and 2mg mL-1 and BD 50% for quinic acid 3mg mL-1. Weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometime negatively affected by the presence of the other compounds.

Components in Lentinus edodes mushroom with anti-biofilm activity directed against bacteria involved in caries and gingivitis

Carla Pruzzo;
2018

Abstract

The present research investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine 5mg mL-1 both in the biofilm inhibition (BI 50%) and in the biofilm disaggregation tests (BD 20%). Considering A. naeslundi, BI values close to 80% were registered for oxalic acid at 1mg mL-1 and 2mg mL-1 and BD 50% for quinic acid 3mg mL-1. Weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometime negatively affected by the presence of the other compounds.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11567/912185
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