Abstract image Two-photon microscopy is the tool of choice for fluorescence imaging of deep tissues with high resolution, but can be limited in three-dimensional acquisition speed and penetration depth. In this work, these issues are addressed by using an acoustic optofluidic lens capable of ultrafast beam shaping on a pixel basis. Driving the lens with different phase profiles enables high-speed volumetric imaging, or enhanced signal-to-background for deeper penetration. Further details can be found in the article by Simonluca Piazza et al.(e201700050)
Inside Front Cover: Enhanced volumetric imaging in 2-photon microscopy via acoustic lens beam shaping (J. Biophotonics 2/2018)
Piazza, Simonluca;Bianchini, Paolo;Diaspro, Alberto;Duocastella, Martí
2018-01-01
Abstract
Abstract image Two-photon microscopy is the tool of choice for fluorescence imaging of deep tissues with high resolution, but can be limited in three-dimensional acquisition speed and penetration depth. In this work, these issues are addressed by using an acoustic optofluidic lens capable of ultrafast beam shaping on a pixel basis. Driving the lens with different phase profiles enables high-speed volumetric imaging, or enhanced signal-to-background for deeper penetration. Further details can be found in the article by Simonluca Piazza et al.(e201700050)
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