Mueller matrix signature in advanced fluorescence microscopy imaging

Nirmal, M.; Qui, J.; Kao, F.; Diaspro, ALBERTO GIOVANNI

doi:10.1088/2040-8986/aa5114

We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes–Mueller polarization microscopy. Thus, Lu–Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.

Mueller matrix signature in advanced fluorescence microscopy imaging

Nirmal, M.;Qui, J.;Kao, F.;DIASPRO, ALBERTO GIOVANNI

2017-01-01

Abstract

We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes–Mueller polarization microscopy. Thus, Lu–Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.