Alyssoides utriculata (L.) Medik. (Brassicaceae) is a perennial evergreen shrub, distributed in the Northeastern Mediterranean area and in several Italian regions. A. utriculata is a nickelhyperaccumulator and it is potentially important for the phytoremediation of metalcontaminated soils. To obtain information on genetic variability of this species the Amplified Fragment Lenght Polymorphism (AFLP) markers were used to analyse samples of two populations of A. utriculata in the Liguria Region (Italy). The first population, identified in Libiola locality (Sestri Levante, GE), grows on a metal-rich soil with anthropic cocontaminations (Fe-Cu-sulphide mining). The second population, identified in Faiallo locality (Genoa), grows on a natural metalliferous soils (viz. Serpentine soil).Eleven DNAs were extracted from 6 and 5 samples of “Libiola” (Lib) and “Faiallo” (FA) population respectively, and analysed using 5 selected AFLP primer combinations. Amplification products were run on denaturating polyacrylamide gels that were stained with silver nitrate. One hundred and eight polymorphic fragments were scored and used for computing the genetic similarities by the Simple Matching coefficient. The similarity values were used to infer in cluster analyses phylogenetic trees according to the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). All genotypes were clearly distinguished by their molecular fingerprints and the Simple Matching genetic similarities index ranged from a minimum of 0.643 between one Lib sample and one FA sample, to a maximum of 0.913 between two FA samples. The UPGMA derived tree separated all Lib samples from samples in two main branches. These preliminary results are indicative of genetic diversity and isolation between “Libiola” and “Faiallo” populations. Further analyses will be necessary to correlate genetic diversity and Nickel hyperaccumulating capacities of these two populations.
Analisi AFLP di Alyssoides utriculata (L.) Medik., specie iperaccumulatrice di nichel AFLP analysis of sub-Mediterranean Nickel-hyperaccumulator Alyssoides utriculata (L.) Medik.
Bazzicalupo, M.;ROCCOTIELLO, ENRICA;MARIOTTI, MAURO
2014-01-01
Abstract
Alyssoides utriculata (L.) Medik. (Brassicaceae) is a perennial evergreen shrub, distributed in the Northeastern Mediterranean area and in several Italian regions. A. utriculata is a nickelhyperaccumulator and it is potentially important for the phytoremediation of metalcontaminated soils. To obtain information on genetic variability of this species the Amplified Fragment Lenght Polymorphism (AFLP) markers were used to analyse samples of two populations of A. utriculata in the Liguria Region (Italy). The first population, identified in Libiola locality (Sestri Levante, GE), grows on a metal-rich soil with anthropic cocontaminations (Fe-Cu-sulphide mining). The second population, identified in Faiallo locality (Genoa), grows on a natural metalliferous soils (viz. Serpentine soil).Eleven DNAs were extracted from 6 and 5 samples of “Libiola” (Lib) and “Faiallo” (FA) population respectively, and analysed using 5 selected AFLP primer combinations. Amplification products were run on denaturating polyacrylamide gels that were stained with silver nitrate. One hundred and eight polymorphic fragments were scored and used for computing the genetic similarities by the Simple Matching coefficient. The similarity values were used to infer in cluster analyses phylogenetic trees according to the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). All genotypes were clearly distinguished by their molecular fingerprints and the Simple Matching genetic similarities index ranged from a minimum of 0.643 between one Lib sample and one FA sample, to a maximum of 0.913 between two FA samples. The UPGMA derived tree separated all Lib samples from samples in two main branches. These preliminary results are indicative of genetic diversity and isolation between “Libiola” and “Faiallo” populations. Further analyses will be necessary to correlate genetic diversity and Nickel hyperaccumulating capacities of these two populations.
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