There are basically two types of microscope, which we call conventional and scanning. The former type is a full-field imaging system. In the latter type, the object is illuminated with a probe beam, and a signal detected. We can generalize the probe to a patterned illumination. Similarly we can generalize the detection to a patterned detection. Combining these we get a range of different modalities: confocal microscopy, structured illumination (with full-field imaging), spinning disk (with multiple illumination points), and so on. The combination allows the spatial frequency bandwidth of the system to be doubled. In general we can record a four dimensional (4D) image of a 2D object (or a 6D image from a 3D object, using an acoustic tuneable lens). The optimum way to directly reconstruct the resulting image is by image scanning microscopy (ISM). But the 4D image is highly redundant, so deconvolution-based approaches are also relevant.
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Titolo: | Microscopy using source and detector arrays |
Autori: | |
Data di pubblicazione: | 2016 |
Serie: | |
Citazione: | Microscopy using source and detector arrays / Sheppard, C.J.R.; Castello, M.; Vicidomini, G.; Duocastella, M.; Diaspro, A. - STAMPA. - (2016), pp. 971302-971302. ((Intervento presentato al convegno Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XXIII. |
Handle: | http://hdl.handle.net/11567/841593 |
ISBN: | 978-1-62841-947-4 |
Appare nelle tipologie: | 04.01 - Contributo in atti di convegno |