We present our results about the investigation of the overall proteome alterations of Chinese Hamster Ovary Cells (CHO) after reverse transformation induced by cyclic 3’,5’-monophosphate adenosine led coupling both 1D and 2 D SDS-PGE and Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) 9Mass Spectrometry. In the same time we analyzed the effectiveness and limitation of such approach, usually adopted for protein separation and identification, in the case of very complex samples as eukaryotic cells. We performed a subcellular fractionation to separate the whole cellular proteome in four fractions in order to reduce proteome complexity and to facilitate subsequent proteome analysis. A new unique pattern of protein synthesis and processing emerged after reverse transformation involving all four cellular compartments but, at the same time, it appeared clear that the approach adopted to study such a complex biological system is not enough powerful to allow the identification of all the involved proteins.

CHO Proteome Alterations Induced by Reverse Transformation

SPERA, ROSANNA;NICOLINI, CLAUDIO
2011

Abstract

We present our results about the investigation of the overall proteome alterations of Chinese Hamster Ovary Cells (CHO) after reverse transformation induced by cyclic 3’,5’-monophosphate adenosine led coupling both 1D and 2 D SDS-PGE and Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) 9Mass Spectrometry. In the same time we analyzed the effectiveness and limitation of such approach, usually adopted for protein separation and identification, in the case of very complex samples as eukaryotic cells. We performed a subcellular fractionation to separate the whole cellular proteome in four fractions in order to reduce proteome complexity and to facilitate subsequent proteome analysis. A new unique pattern of protein synthesis and processing emerged after reverse transformation involving all four cellular compartments but, at the same time, it appeared clear that the approach adopted to study such a complex biological system is not enough powerful to allow the identification of all the involved proteins.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11567/550126
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