"Proteomineering": has the mine been excavated?

The combinatorial peptide ligand library (CPLL) in association with mass spectrometry (MS) can greatly enhance the dynamic range of the analysis of low and very low-abundance proteins constituting the vast majority of species in any sample. When compared with untreated samples, the increment in detection of low-abundance species appears to be at least four-fold. Recently, CPLL have been challenged, however it has been clearly demonstrated in the evaluated paper that the protocols for elution of the captured polypeptides make the difference. Therefore, the solid-phase ligand library made of hexapeptides, remains a promising unique tool for biomarker discovery.