The polydeoxyribonucleotide (PDRN) fraction is an ext. which forms the active component in a new formulation of the drug Placentex (a tissue repair stimulating agent), obtained from human placenta through an original proprietary extn. method.  From a comparison of the UV, NMR and IR spectra of this fraction (before and after nuclease treatment) with that of a similar std. (Sigma D1501), it was shown that the active substances in the PDRN fraction mainly consist of a mixt. of DNA fragments.  By gel electrophoresis, the mol. wts. of the DNA fragments were shown to range from 50 to 2000 base pairs.  Finally, an HPLC method is described, based on an anion-exchange material capable of detg. the amt. of PDRN in different batches of the ext., which varied from 80 to 90%

Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent.

MAZZEI, MAURO;BALBI, ALESSANDRO
1996-01-01

Abstract

The polydeoxyribonucleotide (PDRN) fraction is an ext. which forms the active component in a new formulation of the drug Placentex (a tissue repair stimulating agent), obtained from human placenta through an original proprietary extn. method.  From a comparison of the UV, NMR and IR spectra of this fraction (before and after nuclease treatment) with that of a similar std. (Sigma D1501), it was shown that the active substances in the PDRN fraction mainly consist of a mixt. of DNA fragments.  By gel electrophoresis, the mol. wts. of the DNA fragments were shown to range from 50 to 2000 base pairs.  Finally, an HPLC method is described, based on an anion-exchange material capable of detg. the amt. of PDRN in different batches of the ext., which varied from 80 to 90%
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/382131
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