Expression of the DNAM-1 ligands Nectin-2 (CD112) and poliovirus receptor (CD155) on dendritic cells: relevance for natural killer/dendritic cells interaction

Pende, D; Castriconi, Roberta; Romagnani, P; Spaggiari, GRAZIA MARIA; Marcenaro, S; Dondero, Alessandra; Lazzari, E; Lasagni, L; Martini, S; Rivera, P; Capobianco, A; Moretta, Lorenzo; Moretta, Alessandro; Bottino, Cristina

doi:10.1182/blood-2005-07-2696

In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.

Expression of the DNAM-1 ligands Nectin-2 (CD112) and poliovirus receptor (CD155) on dendritic cells: relevance for natural killer/dendritic cells interaction

PENDE D;CASTRICONI, ROBERTA;ROMAGNANI P;SPAGGIARI, GRAZIA MARIA;MARCENARO S;DONDERO, ALESSANDRA;LAZZARI E;LASAGNI L;MARTINI S;RIVERA P;CAPOBIANCO A;MORETTA, LORENZO;MORETTA, ALESSANDRO;BOTTINO, CRISTINA

2006-01-01

Abstract

In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.