Salvia miniata Fernald (Lamiaceae): characterization of a new clerodane diterpenoid and phytotoxic activity of previously isolated diterpenes

Planta Med 2009; 75 DOI: 10.1055/s-0029-1234884 Salvia miniata Fernald (Lamiaceae): characterization of a new clerodane diterpenoid and phytotoxic activity of previously isolated diterpenes A Bisio 1, D Fraternale 2, E Russo 1, G Romussi 1, S Cafaggi 1, G Caviglioli 1, N De Tommasi 3 1 Dipartimento di Chimica e Tecnologie Farmaceutiche e Alimentari, Università di Genova, Via Brigata Salerno, 16147 Genova, Italia 2 Istituto di Botanica, Università di Urbino, Via Bramante 28, 61029 Urbino, Italia 3 Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via ponte Don Melillo, 84084 Salerno, Italia In a preliminary test, the aerial part exudate of Salvia miniata Fernald [1] showed anti-germinative activity against Papaver rhoeas L. and Avena sativa L. In this work, the antigerminative activity of previously isolated diterpenes [2] has been evaluated and the phytotoxicity (total germination inhibition at 60mg/L against Papaver and at 80mg/L against Avena) of one of these is described. Moreover, we report a new compound obtained from the chromatographic separation of a not previously considered exudate fraction. The surface exudate, obtained by rinsing the plant material with CH2Cl2, and subjected to repeated column chromatography on Sephadex LH-20 and silica gel and to semi-preparative reversed-phase HPLC, yielded a new clerodane diterpenoid (1), identified by IR and NMR analysis, including TOCSY, COSY, HSQC, HMBC and ROESY experiments. References: [1] Epling, C. (1940) A Revision of Salvia, subgenus Calosphace. In: Repertorium Specierum Novarum Regni Vegetabilis. Vol.110. Fedde F. Berkley, California: University of California Press. [2] Bisio, A. et al. (2008) Planta Med. 74:1041-1041