Previous studies showed the presenceof both cholinesterase (ChE) activity, able to hydrolyze the propionyl thiocholine iodide substrate, and "true" acetylcholinesterase (AChE) activity, similar to that of Electrophorus electricus in single-cell amoebae Dictyostelium discoideum. These activities are sensitive to anti-ChE agents, and to the inhibitors classically used to discriminate different ChEs. In this work, we have investigated the presence of ChE activities and of both acetylcholine (ACh) nicotinic and muscarinic receptors, during the developmental cycle of D. discoideum. The spectrophotometric evaluation showed that slugs displayed the highest enzyme activities, AChE being the main activity, while fruiting bodies exhibited the lowest. The presence of AChE activity was cytochemically detected in all the cell populations of the developmental cycle, except in the fruiting bodies where it was localized exclusively to the level of the spores. Molecules recognized by the anti-butyrylcholinesterase antibody were detected in single-cell amoebae, first fingers, slugs, and culminants, except in the fruiting bodies where they were found exclusively in the spore walls. The ACh nicotinic receptors were cytochemically identified in single-cell amoebae, slugs, and spores; however, molecules immunologically related to the ACh muscarinic receptors were not present in the spores.

Detection of cholinesterase activities and acetylcholine receptors during the developmental cycle of Dictyostelium discoideum

AMAROLI, ANDREA;GALLUS, LORENZO;PASSALACQUA, MARIO;FALUGI, CARLA;
2003

Abstract

Previous studies showed the presenceof both cholinesterase (ChE) activity, able to hydrolyze the propionyl thiocholine iodide substrate, and "true" acetylcholinesterase (AChE) activity, similar to that of Electrophorus electricus in single-cell amoebae Dictyostelium discoideum. These activities are sensitive to anti-ChE agents, and to the inhibitors classically used to discriminate different ChEs. In this work, we have investigated the presence of ChE activities and of both acetylcholine (ACh) nicotinic and muscarinic receptors, during the developmental cycle of D. discoideum. The spectrophotometric evaluation showed that slugs displayed the highest enzyme activities, AChE being the main activity, while fruiting bodies exhibited the lowest. The presence of AChE activity was cytochemically detected in all the cell populations of the developmental cycle, except in the fruiting bodies where it was localized exclusively to the level of the spores. Molecules recognized by the anti-butyrylcholinesterase antibody were detected in single-cell amoebae, first fingers, slugs, and culminants, except in the fruiting bodies where they were found exclusively in the spore walls. The ACh nicotinic receptors were cytochemically identified in single-cell amoebae, slugs, and spores; however, molecules immunologically related to the ACh muscarinic receptors were not present in the spores.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/247581
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