In this work a new technique is presented for the formation of black lipid membranes from a single monolayer of bipolar lipids at the air/water interface. The lipid, extracted from the thermophilic archaeobacterium Sulfolobus solfataricus, is characterized by two different polar heads. The membrane is formed with a technique similar to that introduced by Mental and Mueller; however, the lipid is spread only on one side of the teflon partition. Conductance in the presence of valinomycin, voltage-dependent capacitance, current-voltage measurements and electroporation indicate that, as expected, the membrane is asymmetric.
Asymmetric black membranes formed by one monolayer of bipolar lipids at the air-water interface
GLIOZZI, ALESSANDRA;ROBELLO, MAURO;RELINI, ANNALISA;
1994-01-01
Abstract
In this work a new technique is presented for the formation of black lipid membranes from a single monolayer of bipolar lipids at the air/water interface. The lipid, extracted from the thermophilic archaeobacterium Sulfolobus solfataricus, is characterized by two different polar heads. The membrane is formed with a technique similar to that introduced by Mental and Mueller; however, the lipid is spread only on one side of the teflon partition. Conductance in the presence of valinomycin, voltage-dependent capacitance, current-voltage measurements and electroporation indicate that, as expected, the membrane is asymmetric.
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