Influence of polyunsaturated fatty acid enrichment and CYP1A1 induction on lipid peroxidation in tumor cells

We thus applied only one prooxidant stimulus after enriching two hepatoma cell lines (MH1C1 and 7777) with lower concentrations of arachidonic acid (ARA) than those used in previous work, but still able to enhance lipid peroxidation without loss of viability. After pretreatment with ARA and exposure to the prooxidant, the cultured cells were refed with normal growth medium and the effects on the enzymes monitored. Moreover, since cytochrome P450 is also able to influence lipid peroxidation during its catalytic cicle [21], we pretreated the cells with the inducer of cytochrome P450 b-naphthoflavone (BNF) or with both ARA plus BNF, in order to see whether different levels of lipid peroxidation might be achieved. hepatoma cells can be said to adapt to oxidative stress when a single short oxidative stimulus is administered. This adaptation is achieved by decreasing the generation of reactive oxygen species through the inhibition of xenobiotic bioactivation