Lignocellulose-based bamboo powder was used as pristine support to immobilize two different biocatalysts: Candida Antarctica lipase B and Rhizomucor miehei lipase. Both, IB-CalB and IB-RM, physically absorbed on bamboo carrier presented considerable recycling efficiency, up to 10 consecutive cycles, in batch mode for the ethyl palmitate synthesis with conversion about 50 and 80 %, respectively. The kinetic resolution of racemic-1-phenylethanol mediated by IB-CalB showed a higher conversion against the IB-RM in batch mode. IB-CalB packed into a glass column reactor was used to carry on a kinetic resolution in flow mode regime with an intensification conversion up to 45 % with 99 % e.e. The easy and low-cost method of the lipase adsorption without pre-treatment of the carrier can be promising for industrial biocatalytic transformation and immobilization strategy.
Immobilization of lipases on lignocellulosic bamboo powder for biocatalytic transformations in batch and continuous flow
Ginoble Pandoli O
2021-01-01
Abstract
Lignocellulose-based bamboo powder was used as pristine support to immobilize two different biocatalysts: Candida Antarctica lipase B and Rhizomucor miehei lipase. Both, IB-CalB and IB-RM, physically absorbed on bamboo carrier presented considerable recycling efficiency, up to 10 consecutive cycles, in batch mode for the ethyl palmitate synthesis with conversion about 50 and 80 %, respectively. The kinetic resolution of racemic-1-phenylethanol mediated by IB-CalB showed a higher conversion against the IB-RM in batch mode. IB-CalB packed into a glass column reactor was used to carry on a kinetic resolution in flow mode regime with an intensification conversion up to 45 % with 99 % e.e. The easy and low-cost method of the lipase adsorption without pre-treatment of the carrier can be promising for industrial biocatalytic transformation and immobilization strategy.
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