Since new antibacterial agents against multi-drug resistant (MDR) bacteria are urgently needed, we recently synthetized cationic dendrimers and copolymers and assessed their antibacterial activity on numerous MDR clinical isolates. Being cationic, the prepared macromolecules electrostatically interacted with pathogens’ surfaces, causing irreversible damages and rapid bacterial death. A lysine dendrimer having 192 cationic groups (N+) was strongly active preferentially on non-fermenting Gram-negative species, displaying MICs comparable to colistin against P. aeruginosa (2.1 µM). A lysine dendrimer (128 N+) was explicitly active on Acinetobacter, while a cationic copolymer showed remarkable antibacterial activity against numerous Gram-positive and Gram-negative species. In 24 h-time-killing experiments, all of the mentioned macromolecules displayed rapid bactericidal effects, while when tested on human keratinocytes, especially G5-PDK, showed low levels of cytotoxicity and high values of selectivity indices. Due to their physicochemical properties and bactericidal potency, the herein reviewed cationic macromolecules could represent novel tools for realizing either a targeted or a broad-spectrum bactericidal action, regardless of the bacterial resistance to current antibiotics.

Biocidal Cationic Macromolecules Irrespective of Bacterial Resistance: Our Best Achievements

ALFEI SILVANA;PIATTI GABRIELLA;CAVIGLIA DEBORA;SCHITO GIAN CARLO;ZUCCARI GUENDALINA;SCHITO ANNA MARIA
2022

Abstract

Since new antibacterial agents against multi-drug resistant (MDR) bacteria are urgently needed, we recently synthetized cationic dendrimers and copolymers and assessed their antibacterial activity on numerous MDR clinical isolates. Being cationic, the prepared macromolecules electrostatically interacted with pathogens’ surfaces, causing irreversible damages and rapid bacterial death. A lysine dendrimer having 192 cationic groups (N+) was strongly active preferentially on non-fermenting Gram-negative species, displaying MICs comparable to colistin against P. aeruginosa (2.1 µM). A lysine dendrimer (128 N+) was explicitly active on Acinetobacter, while a cationic copolymer showed remarkable antibacterial activity against numerous Gram-positive and Gram-negative species. In 24 h-time-killing experiments, all of the mentioned macromolecules displayed rapid bactericidal effects, while when tested on human keratinocytes, especially G5-PDK, showed low levels of cytotoxicity and high values of selectivity indices. Due to their physicochemical properties and bactericidal potency, the herein reviewed cationic macromolecules could represent novel tools for realizing either a targeted or a broad-spectrum bactericidal action, regardless of the bacterial resistance to current antibiotics.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11567/1087716
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact