The most common limitations of drug carriers, such as liposomes, are low Encapsulation Efficiency (EE), poor biocompatibility and drug degradation. Several techniques have been developed to overcome these problems; among these, Supercritical assisted Liposome formation (SuperLip) is a CO2 mediated technique for the production of liposomes at nanometric level. SuperLip has been employed to produce amoxicillin loaded liposomes. Vesicles have been loaded with 1, 5, 10 and 20 % (w/w) amoxicillin to lipid ratio; then, 1 % (w/w) cholesterol/lipid was added to liposomes lamellae. Liposomes showed mean diameters of about 200 nm and EE up to 84 %. Anti-microbial effect of Amoxicillin-loaded liposomes on Escherichia coli was studied. The growth of E.coli was monitored inoculating bacteria in Luria-Bertani medium with different liposomes concentrations (25, 50, 100 ppm) and different antibiotic/lipid ratio (5, 10, 20 %). An increase in the amoxicillin/lipid ratio enhanced growth inhibition of E.coli, especially at 100 ppm.

A supercritical assisted process for the production of amoxicillin loaded liposomes for anti-microbial applications

Ferrari P. F.;Campardelli R.;Perego P.
2020-01-01

Abstract

The most common limitations of drug carriers, such as liposomes, are low Encapsulation Efficiency (EE), poor biocompatibility and drug degradation. Several techniques have been developed to overcome these problems; among these, Supercritical assisted Liposome formation (SuperLip) is a CO2 mediated technique for the production of liposomes at nanometric level. SuperLip has been employed to produce amoxicillin loaded liposomes. Vesicles have been loaded with 1, 5, 10 and 20 % (w/w) amoxicillin to lipid ratio; then, 1 % (w/w) cholesterol/lipid was added to liposomes lamellae. Liposomes showed mean diameters of about 200 nm and EE up to 84 %. Anti-microbial effect of Amoxicillin-loaded liposomes on Escherichia coli was studied. The growth of E.coli was monitored inoculating bacteria in Luria-Bertani medium with different liposomes concentrations (25, 50, 100 ppm) and different antibiotic/lipid ratio (5, 10, 20 %). An increase in the amoxicillin/lipid ratio enhanced growth inhibition of E.coli, especially at 100 ppm.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/1074961
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