Pathways of Endocrine Disruption in the larval development of the mediterranean mussel Mytilus galloprovincialis

Endocrine Disruptors (EDCs) are a class of anthropogenic environmental contaminants that affect the endocrine systems of living organisms. The lack of knowledge of endocrine systems prevents the understanding of the effect of PEs in invertebrates. Accordingly, this thesis aims to address the issue of endocrine disruption in marine invertebrates by exploiting the larval development of the Mediterranean mussel Mytilus galloprovincialis. By applying the logic of the Adverse Outcome Pathway-AOP, the work in this thesis attempted to characterize the mechanism of action of the model EDCs BPA, TBBPA and TBT in mussel larvae and to identify plausible neuroendocrine pathways by which they induce their deleterious biological effect. The neuroendocrine elements regulating the biogenesis of the larval shell have been characterized and the morphogenetic process established as sensitive to neuroendocrine disruption. Plausible neuroendocrine AOPs that could be linked to the biological effects of the model EDCs have been identified. In addition, the possible AOP of TBT has established a plausible relationship between nuclear receptor signaling and the neuroendocrine system of mussel larvae. The results of this thesis represent essential evidence that will help and advance the current understanding of endocrine disruption mechanisms in invertebrate organisms.