LANZANO', LUCA
LANZANO', LUCA
3 Color - 3 Dimensional STED Nanoscopy
2015-01-01 Peres, C.; Oneto, M.; D’Autilia, F.; Galiani, S.; Lanzano, L.; Vicidomini, G.; Diaspro, A.; Bianchini, P.
A Novel Viewpoint to Analyze Structured Illumination Microscopy (Sim) Data
2019-01-01 Cainero, Isotta; Pelicci, Simone; Di Bona, Melody; Diaspro, Alberto; Lanzano', Luca
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
2019-01-01 Castello, M.; Tortarolo, G.; Buttafava, M.; Deguchi, T.; Villa, F.; Koho, S.; Pesce, L.; Oneto, M.; Pelicci, S.; Lanzano, L.; Bianchini, P.; Sheppard, C. J. R.; Diaspro, A.; Tosi, A.; Vicidomini, G.
Advances in Gated CW STED Microscopy: Toward a Versatile Implementation
2016-01-01 Hernández, Iván Coto; Castello, Marco; Lanzano, Luca; Diaspro, Alberto; Vicidomini, Giuseppe
Airyscan Cca Provides Structural and Dynamics Fingerprinting of Subcellular Compartments in Living Cells
2019-01-01 Scipioni, Lorenzo; Lanzano′, Luca; Diaspro, Alberto; Gratton, Enrico
Background-Free Super-Resolution Microscopy of Subcellular Structures by Lifetime Tuning and Photons Separation
2015-01-01 Lanzano, L.; Coto Hernandez, I.; Castello, M.; Gratton, E.; Diaspro, A.; Vicidomini, G.
Chromatin Alterations in a Model of Oncogene Activation Studied by Advanced Fluorescence Microscopy
2019-01-01 Lanzano′, Luca; Oneto, Michele; Cainero, Isotta; Pelicci, Simone; Sarmento, Maria; Scipioni, Lorenzo; Faretta, Mario; Furia, Laura; Ivan Dellino, Gaetano; Giuseppe Pelicci, Pier; Bianchini, Paolo; Diaspro, Alberto
Chromatin Nanoscale Organization Investigated by FLIM-FRET and STED Superresolution Microscopy
2019-01-01 Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Cainero, Isotta; Barboro, Paola; Diaspro, Alberto; Lanzano′, Luca
Combining Expansion Microscopy and STED Nanoscopy for the Study of Cellular Organization
2017-01-01 Cainero, I.; Oneto, M.; Pesce, L.; Zanini, G.; Lanzanò, L.; Diaspro, A.; Bianchini, P
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector
2018-01-01 Scipioni, L.; Lanzano, L.; Diaspro, A.; Gratton, E.
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells.
2018-01-01 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
Improving multiphoton STED nanoscopy with separation of photons by LIfetime Tuning (SPLIT)
2018-01-01 Tortarolo, Giorgio; Vicidomini, Giuseppe; Diaspro, Alberto; Jowett, Nate; Castello, Marco; Lanzanò, Luca; Coto Hernández, Iván
Intensity Sorted Fluorescence Correlation Spectroscopy: A Novel Method to Probe Nuclear Dynamics and Chromatin Organization in Living Cells
2019-01-01 Di Bona, Melody; Pelicci, Simone; Cainero, Isotta; Vicidomini, Giuseppe; Mazza, Davide; Mancini, Michael A.; Diaspro, Alberto; Lanzano', Luca
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus
2018-01-01 Diaspro, Alberto; Anthony, Nicholas; Bianchini, Paolo; Cainero, Isotta; Di Bona, Melody; Lanzanò, Luca; Le Gratiet, Aymeric; Marongiu, Riccardo; Oneto, Michele; Pelicci, Simone; Pesce, Luca
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
2017-01-01 Lanzanò, Luca; Scipioni, Lorenzo; Di Bona, Melody; Bianchini, Paolo; Bizzarri, Ranieri; Cardarelli, Francesco; Diaspro, Alberto; Vicidomini, Giuseppe
Measuring expansion from macro- to nanoscale using NPC as intrinsic reporter
2019-01-01 Pesce, Luca; Cozzolino, Marco; Lanzanò, Luca; Diaspro, Alberto; Bianchini, Paolo
Measuring Mobility in Chromatin by Intensity-Sorted FCS
2019-01-01 Di Bona, M.; Mancini, M. A.; Mazza, D.; Vicidomini, G.; Diaspro, A.; Lanzano, L.
Photon-separation to enhance the spatial resolution of pulsed STED microscopy
2019-01-01 Tortarolo, G.; Sun, Y.; Teng, K. W.; Ishitsuka, Y.; Lanzano, L.; Selvin, P. R.; Barbieri, B.; Diaspro, A.; Vicidomini, G.
STED microscopy: Exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities
2018-01-01 Lanzano, L.; Vicidomini, G.; Scipioni, L.; Castello, M.; Diaspro, A.
The Nucluear Pore Complex as Intrinsic Reporter for Isotropic Expansion Microscopy
2019-01-01 Bianchini, Paolo; Pesce, Luca; Cozzolino, Marco; Lanzano', Luca; Diaspro, Alberto