LANZANO', LUCA

LANZANO', LUCA  

100023 - Dipartimento di Informatica, bioingegneria, robotica e ingegneria dei sistemi  

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Risultati 1 - 20 di 22 (tempo di esecuzione: 0.069 secondi).
Titolo Data di pubblicazione Autore(i) File
3 Color - 3 Dimensional STED Nanoscopy 1-gen-2015 Peres, C.; Oneto, M.; D’Autilia, F.; Galiani, S.; Lanzano, L.; Vicidomini, G.; Diaspro, A.; Bianchini, P.
Advances in Gated CW STED Microscopy: Toward a Versatile Implementation 1-gen-2016 Hernández, Iván Coto; Castello, Marco; Lanzano, Luca; Diaspro, Alberto; Vicidomini, Giuseppe
Airyscan Cca Provides Structural and Dynamics Fingerprinting of Subcellular Compartments in Living Cells 1-gen-2019 Scipioni, Lorenzo; Lanzano′, Luca; Diaspro, Alberto; Gratton, Enrico
Background-Free Super-Resolution Microscopy of Subcellular Structures by Lifetime Tuning and Photons Separation 1-gen-2015 Lanzano, L.; Coto Hernandez, I.; Castello, M.; Gratton, E.; Diaspro, A.; Vicidomini, G.
Chromatin Alterations in a Model of Oncogene Activation Studied by Advanced Fluorescence Microscopy 1-gen-2019 Lanzano′, Luca; Oneto, Michele; Cainero, Isotta; Pelicci, Simone; Sarmento, Maria; Scipioni, Lorenzo; Faretta, Mario; Furia, Laura; Ivan Dellino, Gaetano; Giuseppe Pelicci, Pier; Bianchini, Paolo; Diaspro, Alberto
Chromatin Nanoscale Organization Investigated by FLIM-FRET and STED Superresolution Microscopy 1-gen-2019 Pelicci, Simone; Oneto, Michele; Di Bona, Melody; Cainero, Isotta; Barboro, Paola; Diaspro, Alberto; Lanzano′, Luca
Combining Expansion Microscopy and STED Nanoscopy for the Study of Cellular Organization 1-gen-2017 Cainero, I.; Oneto, M.; Pesce, L.; Zanini, G.; Lanzanò, L.; Diaspro, A.; Bianchini, P
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector 1-gen-2018 Scipioni, L.; Lanzano, L.; Diaspro, A.; Gratton, E.
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells. 1-gen-2018 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
Improving multiphoton STED nanoscopy with separation of photons by LIfetime Tuning (SPLIT) 1-gen-2018 Tortarolo, Giorgio; Vicidomini, Giuseppe; Diaspro, Alberto; Jowett, Nate; Castello, Marco; Lanzanò, Luca; Coto Hernández, Iván
Intensity Sorted Fluorescence Correlation Spectroscopy: A Novel Method to Probe Nuclear Dynamics and Chromatin Organization in Living Cells 1-gen-2019 Di Bona, Melody; Pelicci, Simone; Cainero, Isotta; Vicidomini, Giuseppe; Mazza, Davide; Mancini, Michael A.; Diaspro, Alberto; Lanzano', Luca
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus 1-gen-2018 Diaspro, Alberto; Anthony, Nicholas; Bianchini, Paolo; Cainero, Isotta; Di Bona, Melody; Lanzanò, Luca; Le Gratiet, Aymeric; Marongiu, Riccardo; Oneto, Michele; Pelicci, Simone; Pesce, Luca
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS 1-gen-2017 Lanzanò, Luca; Scipioni, Lorenzo; Di Bona, Melody; Bianchini, Paolo; Bizzarri, Ranieri; Cardarelli, Francesco; Diaspro, Alberto; Vicidomini, Giuseppe
Measuring expansion from macro- to nanoscale using NPC as intrinsic reporter 1-gen-2019 Pesce, Luca; Cozzolino, Marco; Lanzanò, Luca; Diaspro, Alberto; Bianchini, Paolo
Measuring Mobility in Chromatin by Intensity-Sorted FCS 1-gen-2019 Di Bona, M.; Mancini, M. A.; Mazza, D.; Vicidomini, G.; Diaspro, A.; Lanzano, L.
A Novel Viewpoint to Analyze Structured Illumination Microscopy (Sim) Data 1-gen-2019 Cainero, Isotta; Pelicci, Simone; Di Bona, Melody; Diaspro, Alberto; Lanzano', Luca
The Nucluear Pore Complex as Intrinsic Reporter for Isotropic Expansion Microscopy 1-gen-2019 Bianchini, Paolo; Pesce, Luca; Cozzolino, Marco; Lanzano', Luca; Diaspro, Alberto
Photon-separation to enhance the spatial resolution of pulsed STED microscopy 1-gen-2019 Tortarolo, G.; Sun, Y.; Teng, K. W.; Ishitsuka, Y.; Lanzano, L.; Selvin, P. R.; Barbieri, B.; Diaspro, A.; Vicidomini, G.
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM 1-gen-2019 Castello, M.; Tortarolo, G.; Buttafava, M.; Deguchi, T.; Villa, F.; Koho, S.; Pesce, L.; Oneto, M.; Pelicci, S.; Lanzano, L.; Bianchini, P.; Sheppard, C. J. R.; Diaspro, A.; Tosi, A.; Vicidomini, G.
The SPLIT approach for enhancing the spatial resolution in pulsed STED microscopy with FastFLIM and phasor plots 1-gen-2019 Tortarolo, G.; Sun, Y.; Shah, S.; Barbieri, B.; Teng, K. -W.; Ishitsuka, Y.; Selvin, P. R.; Lanzano, L.; Diaspro, A.; Vicidomini, G.