LANZANO', LUCA
LANZANO', LUCA
3 Color - 3 Dimensional STED Nanoscopy
2015-01-01 Peres, C.; Oneto, M.; D’Autilia, F.; Galiani, S.; Lanzano, L.; Vicidomini, G.; Diaspro, A.; Bianchini, P.
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
2019-01-01 Castello, M.; Tortarolo, G.; Buttafava, M.; Deguchi, T.; Villa, F.; Koho, S.; Pesce, L.; Oneto, M.; Pelicci, S.; Lanzano, L.; Bianchini, P.; Sheppard, C. J. R.; Diaspro, A.; Tosi, A.; Vicidomini, G.
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector
2018-01-01 Scipioni, L.; Lanzano, L.; Diaspro, A.; Gratton, E.
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells.
2018-01-01 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus
2018-01-01 Diaspro, Alberto; Anthony, Nicholas; Bianchini, Paolo; Cainero, Isotta; Di Bona, Melody; Lanzanò, Luca; Le Gratiet, Aymeric; Marongiu, Riccardo; Oneto, Michele; Pelicci, Simone; Pesce, Luca
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
2017-01-01 Lanzanò, Luca; Scipioni, Lorenzo; Di Bona, Melody; Bianchini, Paolo; Bizzarri, Ranieri; Cardarelli, Francesco; Diaspro, Alberto; Vicidomini, Giuseppe
Measuring expansion from macro- to nanoscale using NPC as intrinsic reporter
2019-01-01 Pesce, Luca; Cozzolino, Marco; Lanzanò, Luca; Diaspro, Alberto; Bianchini, Paolo
Measuring Mobility in Chromatin by Intensity-Sorted FCS
2019-01-01 Di Bona, M.; Mancini, M. A.; Mazza, D.; Vicidomini, G.; Diaspro, A.; Lanzano, L.
Photon-separation to enhance the spatial resolution of pulsed STED microscopy
2019-01-01 Tortarolo, G.; Sun, Y.; Teng, K. W.; Ishitsuka, Y.; Lanzano, L.; Selvin, P. R.; Barbieri, B.; Diaspro, A.; Vicidomini, G.
Three-dimensional multiple-particle tracking with nanometric precision over tunable axial ranges
2017-01-01 Sancataldo, Giuseppe; Scipioni, Lorenzo; Ravasenga, Tiziana; Lanzanò, Luca; Diaspro, Alberto; Barberis, Andrea; Duocastella, Martí