LANZANO', LUCA

LANZANO', LUCA  

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Titolo Data di pubblicazione Autore(i) File
3 Color - 3 Dimensional STED Nanoscopy 1-gen-2015 Peres, C.; Oneto, M.; D’Autilia, F.; Galiani, S.; Lanzano, L.; Vicidomini, G.; Diaspro, A.; Bianchini, P.
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM 1-gen-2019 Castello, M.; Tortarolo, G.; Buttafava, M.; Deguchi, T.; Villa, F.; Koho, S.; Pesce, L.; Oneto, M.; Pelicci, S.; Lanzano, L.; Bianchini, P.; Sheppard, C. J. R.; Diaspro, A.; Tosi, A.; Vicidomini, G.
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector 1-gen-2018 Scipioni, L.; Lanzano, L.; Diaspro, A.; Gratton, E.
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells. 1-gen-2018 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
LIQUITOPY®: A Liquid Tunable Microscope to Study Chromatin Organization in the Cell Nucleus 1-gen-2018 Diaspro, Alberto; Anthony, Nicholas; Bianchini, Paolo; Cainero, Isotta; Di Bona, Melody; Lanzanò, Luca; Le Gratiet, Aymeric; Marongiu, Riccardo; Oneto, Michele; Pelicci, Simone; Pesce, Luca
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS 1-gen-2017 Lanzanò, Luca; Scipioni, Lorenzo; Di Bona, Melody; Bianchini, Paolo; Bizzarri, Ranieri; Cardarelli, Francesco; Diaspro, Alberto; Vicidomini, Giuseppe
Measuring expansion from macro- to nanoscale using NPC as intrinsic reporter 1-gen-2019 Pesce, Luca; Cozzolino, Marco; Lanzanò, Luca; Diaspro, Alberto; Bianchini, Paolo
Measuring Mobility in Chromatin by Intensity-Sorted FCS 1-gen-2019 Di Bona, M.; Mancini, M. A.; Mazza, D.; Vicidomini, G.; Diaspro, A.; Lanzano, L.
Photon-separation to enhance the spatial resolution of pulsed STED microscopy 1-gen-2019 Tortarolo, G.; Sun, Y.; Teng, K. W.; Ishitsuka, Y.; Lanzano, L.; Selvin, P. R.; Barbieri, B.; Diaspro, A.; Vicidomini, G.
Three-dimensional multiple-particle tracking with nanometric precision over tunable axial ranges 1-gen-2017 Sancataldo, Giuseppe; Scipioni, Lorenzo; Ravasenga, Tiziana; Lanzanò, Luca; Diaspro, Alberto; Barberis, Andrea; Duocastella, Martí