Previous studies on the toxicity induced by Ag NPs of different size on zebrafish during development have almost exclusively characterized macroscopic effects, in particular anomalies in the spontaneous movements, hatching rate/time disturbance and the presence of different malformations [1][2]. However little is known about a possible interaction of Ag NPs with sub- cellular structures. In this study we focus our attention on the biological effects of small-sized silver nanoparticles (8-10nm) through a multi-level investigation on zebrafish larvae. The use of these model organisms and the possibility to image them using advanced microscopy techniques represent an optimal opportunity to screen toxicity effects of nanoparticles at microscopic level. In particular, the use of advanced microscopy techniques (such as confocal and selective plane illumination microscopy (SPIM)) [3] [4] allows for 3D high resolution imaging of possible effects of nanoparticles on the cytoskeletal architecture. With this approach high cellular and sub-cellular resolution can be achieved. So far, we have investigated the possible damage induced by Ag NPs in the structure of both tubulin and filamentous actin (F-actin) in zebrafish larvae.
Zebrafish Larvae as Model System to Study Possible Toxicity of Silver Nanoparticles at Cytoskeletal Level by Means of Advanced Microscopy
d’Amora M.;Lavagnino Z.;Sancataldo G.;Diaspro A.
2015-01-01
Abstract
Previous studies on the toxicity induced by Ag NPs of different size on zebrafish during development have almost exclusively characterized macroscopic effects, in particular anomalies in the spontaneous movements, hatching rate/time disturbance and the presence of different malformations [1][2]. However little is known about a possible interaction of Ag NPs with sub- cellular structures. In this study we focus our attention on the biological effects of small-sized silver nanoparticles (8-10nm) through a multi-level investigation on zebrafish larvae. The use of these model organisms and the possibility to image them using advanced microscopy techniques represent an optimal opportunity to screen toxicity effects of nanoparticles at microscopic level. In particular, the use of advanced microscopy techniques (such as confocal and selective plane illumination microscopy (SPIM)) [3] [4] allows for 3D high resolution imaging of possible effects of nanoparticles on the cytoskeletal architecture. With this approach high cellular and sub-cellular resolution can be achieved. So far, we have investigated the possible damage induced by Ag NPs in the structure of both tubulin and filamentous actin (F-actin) in zebrafish larvae.
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