The desire for new methods that can explore the function of biological systems without the need for sample modification, or the addition of fluorescent tags, is steadily emerging. The development of so called ‘label free’ microscopies is important for the exploration of the natural state of biological systems, without alteration and without potentially affecting the processes being explored [1]. Label free techniques utilize contrast from novel processes to provide a means of imaging without external modification. However, the imaging of weakly interacting samples is not trivial and has required significant development or modification of current techniques to produce suitable resolution and contrast.
Label Free Microscopy with Ptychography
Bianchini, Paolo;Diaspro, Alberto
2019-01-01
Abstract
The desire for new methods that can explore the function of biological systems without the need for sample modification, or the addition of fluorescent tags, is steadily emerging. The development of so called ‘label free’ microscopies is important for the exploration of the natural state of biological systems, without alteration and without potentially affecting the processes being explored [1]. Label free techniques utilize contrast from novel processes to provide a means of imaging without external modification. However, the imaging of weakly interacting samples is not trivial and has required significant development or modification of current techniques to produce suitable resolution and contrast.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.