Summary form only given. The combination of the AFM technique and the sphere-mediated microscopy (SMM) [1] opens a new opportunity to the Atomic Force Microscopy (AFM). With the help of a tipless AFM cantilever is possible to place and scan a microspheres (MS) close to the surface. From the optical point of view, when a MS is close to a surface act as high NA nanolenses whose optical characteristics define the maximum attainable resolution.We performed a detailed measurement of the spatial resolution in SMM by imaging a calibration target made of gratings with different periodicity. Images of the test gratings with and without the microsphere allowed a full characterization of the spatial frequency response of our microscope (modulation transfer function or MTF) and the consequent quantitative determination of the enhancement in resolution induced by the microsphere[2].

Microsphere embedded in cantilever opens the AFM to high resolution optical microscopy

Duocastella, Marti;Zaccaria, Remo Proietti;Jacassi, Andrea;Diaspro, Alberto;
2017

Abstract

Summary form only given. The combination of the AFM technique and the sphere-mediated microscopy (SMM) [1] opens a new opportunity to the Atomic Force Microscopy (AFM). With the help of a tipless AFM cantilever is possible to place and scan a microspheres (MS) close to the surface. From the optical point of view, when a MS is close to a surface act as high NA nanolenses whose optical characteristics define the maximum attainable resolution.We performed a detailed measurement of the spatial resolution in SMM by imaging a calibration target made of gratings with different periodicity. Images of the test gratings with and without the microsphere allowed a full characterization of the spatial frequency response of our microscope (modulation transfer function or MTF) and the consequent quantitative determination of the enhancement in resolution induced by the microsphere[2].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11567/919190
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact