. In vitro animal models of the nervous system have been in use for many years provide an important tool for functional studies at cellular and molecular levels. Aim of our study was to generate standardized neural precursor populations from human Adipose-derived Stem Cells (hADSC), by tissue, routinely discarded after liposuction surgeries. hADSC were submitted to neural differentiated procedures, and positiveness for Nestinn and GFAP confirmed their commitment to neural like cells (N-hADSC). To test the suitability of N-hADSC as model for neural toxicity assessment, N-hADSC and rat cortical neuron cells were exposed for 24-48 hours to three neurotoxic compounds ( Carbaryl, Lidocaine and Phenytoin). A different behavior confirm the need of specie-specificity in toxicity tests.
Assessment of neurotoxicity assay based on neural-like cells from human adipose derived stem cells
TIRENDI, SARA;SCANAROTTI, CHIARA;VERNAZZA, STEFANIA;Bassi, A. M.
2017-01-01
Abstract
. In vitro animal models of the nervous system have been in use for many years provide an important tool for functional studies at cellular and molecular levels. Aim of our study was to generate standardized neural precursor populations from human Adipose-derived Stem Cells (hADSC), by tissue, routinely discarded after liposuction surgeries. hADSC were submitted to neural differentiated procedures, and positiveness for Nestinn and GFAP confirmed their commitment to neural like cells (N-hADSC). To test the suitability of N-hADSC as model for neural toxicity assessment, N-hADSC and rat cortical neuron cells were exposed for 24-48 hours to three neurotoxic compounds ( Carbaryl, Lidocaine and Phenytoin). A different behavior confirm the need of specie-specificity in toxicity tests.
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