The aims of this work were to a) characterize the mycobiota present in these colloidal minerals evaluating the presence of alive fungal propagules and to extracting bacteria DNA; b) verify the strains fungal tolerance and bioaccumulation capability on greenish-blue and ZnSO4 enriched media; c) evaluate potential impact of bacteria in the geochemistry of the system. The preliminary results show an interesting and selected mycobiota able to survive under unfavourable environmental conditions. A significant number of fungal strains were isolated in pure culture. Among them, species belonging to Penicillium and Trichoderma genera were tested on both greenish-blue and ZnSO4 enriched media. The results shown a significant tolerance and bioaccumulation capability to some PTEs. The same colloidal precipitates have been processed to extract bacteria DNA, using a specific procedure developed for DNA extraction from sediments. The results gave a good yield of nucleic acids and the positive PCR amplification of 16S rDNA accomplished the first step for future metagenomic analyses.
Colloidal precipitates related to Acid Mine Drainage: bacterial diversity and microfungi heavy metal interactions
LUCCHETTI, GABRIELLA;CARBONE, CRISTINA;CONSANI, SIRIO;ZOTTI, MIRCA;POZZOLINI, MARINA;GIOVINE, MARCO
2015-01-01
Abstract
The aims of this work were to a) characterize the mycobiota present in these colloidal minerals evaluating the presence of alive fungal propagules and to extracting bacteria DNA; b) verify the strains fungal tolerance and bioaccumulation capability on greenish-blue and ZnSO4 enriched media; c) evaluate potential impact of bacteria in the geochemistry of the system. The preliminary results show an interesting and selected mycobiota able to survive under unfavourable environmental conditions. A significant number of fungal strains were isolated in pure culture. Among them, species belonging to Penicillium and Trichoderma genera were tested on both greenish-blue and ZnSO4 enriched media. The results shown a significant tolerance and bioaccumulation capability to some PTEs. The same colloidal precipitates have been processed to extract bacteria DNA, using a specific procedure developed for DNA extraction from sediments. The results gave a good yield of nucleic acids and the positive PCR amplification of 16S rDNA accomplished the first step for future metagenomic analyses.
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