CINECA IRIS Institutional Research Information System
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishThe aims of this work were to a) characterize the mycobiota present in these colloidal minerals evaluating the presence of alive fungal propagules and to extracting bacteria DNA; b) verify the strains fungal tolerance and bioaccumulation capability on greenish-blue and ZnSO4 enriched media; c) evaluate potential impact of bacteria in the geochemistry of the system. The preliminary results show an interesting and selected mycobiota able to survive under unfavourable environmental conditions. A significant number of fungal strains were isolated in pure culture. Among them, species belonging to Penicillium and Trichoderma genera were tested on both greenish-blue and ZnSO4 enriched media. The results shown a significant tolerance and bioaccumulation capability to some PTEs. The same colloidal precipitates have been processed to extract bacteria DNA, using a specific procedure developed for DNA extraction from sediments. The results gave a good yield of nucleic acids and the positive PCR amplification of 16S rDNA accomplished the first step for future metagenomic analyses.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
| Titolo: | Colloidal precipitates related to Acid Mine Drainage: bacterial diversity and microfungi heavy metal interactions |
| Autori: | |
| Data di pubblicazione: | 2015 |
| Abstract: | The aims of this work were to a) characterize the mycobiota present in these colloidal minerals evaluating the presence of alive fungal propagules and to extracting bacteria DNA; b) verify the strains fungal tolerance and bioaccumulation capability on greenish-blue and ZnSO4 enriched media; c) evaluate potential impact of bacteria in the geochemistry of the system. The preliminary results show an interesting and selected mycobiota able to survive under unfavourable environmental conditions. A significant number of fungal strains were isolated in pure culture. Among them, species belonging to Penicillium and Trichoderma genera were tested on both greenish-blue and ZnSO4 enriched media. The results shown a significant tolerance and bioaccumulation capability to some PTEs. The same colloidal precipitates have been processed to extract bacteria DNA, using a specific procedure developed for DNA extraction from sediments. The results gave a good yield of nucleic acids and the positive PCR amplification of 16S rDNA accomplished the first step for future metagenomic analyses. |
| Handle: | http://hdl.handle.net/11567/821946 |
| Appare nelle tipologie: | 04.02 - Abstract in atti di convegno |
File in questo prodotto:
Copyright © 2020