A construct based on the electrostatic layer-by-layer self assembly technique has been fabricated, to be used as a tailored device to encourage nerve regeneration. A multilayered nanocoating composed by three precursor bilayers of cationic and anionic polyelectrolytes followed by bilayers of poly-D-lysine (PDL) and antibody specific to transforming growth factor beta1 (anti-TGF-beta1) has been deposited on HYAFF 11reg. Initially the assembly process has been monitored by quartz crystal microbalance (QCM) in order to select the optimal working conditions for nanocoating deposition. Structural studies of the resulting multilayers confirmed stepwise deposition of anti-TGF-beta1 with an average layer thickness of 2.6 nm and an average layer mass of 117 ng. Atomic force microscopy has been used to characterize multilayer uniformity. Finally, the immunological activity of the multilayered structure has been assessed. The results show that anti-TGF-beta1 can be included in its active form in a predetermined multilayered structure onto HYAFF11reg with quantitative control of layer thickness and weight, providing a high potential tool in tissue engineering
Nanofunctionalisation for the Treatment of Peripheral Nervous System Injuries
PASTORINO, LAURA;CANEVA SOUMETZ, FEDERICO;RUGGIERO, CARMELINA
2005-01-01
Abstract
A construct based on the electrostatic layer-by-layer self assembly technique has been fabricated, to be used as a tailored device to encourage nerve regeneration. A multilayered nanocoating composed by three precursor bilayers of cationic and anionic polyelectrolytes followed by bilayers of poly-D-lysine (PDL) and antibody specific to transforming growth factor beta1 (anti-TGF-beta1) has been deposited on HYAFF 11reg. Initially the assembly process has been monitored by quartz crystal microbalance (QCM) in order to select the optimal working conditions for nanocoating deposition. Structural studies of the resulting multilayers confirmed stepwise deposition of anti-TGF-beta1 with an average layer thickness of 2.6 nm and an average layer mass of 117 ng. Atomic force microscopy has been used to characterize multilayer uniformity. Finally, the immunological activity of the multilayered structure has been assessed. The results show that anti-TGF-beta1 can be included in its active form in a predetermined multilayered structure onto HYAFF11reg with quantitative control of layer thickness and weight, providing a high potential tool in tissue engineering
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