Biomass recycling from four successive bioconversion steps demonstrated the possibility of employing such a resting cell system for the continuous production of vanillin. Among the tested immobilization supports (polyurethane, synthetic sponge and porous glass) the synthetic sponge proved to be the best material in terms of both vanillin formation (Cv = 0.080 g/L) and productivity (Qv = 0.019 g/L h) at the end of entrapment tests. Thus, it was used in continuous bioconversions using a fixed-bed column with immobilized E. coli JM109 (pBB1) cells. The highest vanillin yield (YP/S = 0.851 mol/mol) was obtained at a dilution rate of 0.022 /h.
Conversión microbiana de ácido ferúlico a vainillina
DE FAVERI, DANILO;TORRE, PAOLO;ALIAKBARIAN, BAHAR;PEREGO, PATRIZIA;CONVERTI, ATTILIO
2008-01-01
Abstract
Biomass recycling from four successive bioconversion steps demonstrated the possibility of employing such a resting cell system for the continuous production of vanillin. Among the tested immobilization supports (polyurethane, synthetic sponge and porous glass) the synthetic sponge proved to be the best material in terms of both vanillin formation (Cv = 0.080 g/L) and productivity (Qv = 0.019 g/L h) at the end of entrapment tests. Thus, it was used in continuous bioconversions using a fixed-bed column with immobilized E. coli JM109 (pBB1) cells. The highest vanillin yield (YP/S = 0.851 mol/mol) was obtained at a dilution rate of 0.022 /h.
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