The combination of neuronal networks derived from human induced pluripotent stem cells (h-iPSCs) with micro-electrode arrays (MEAs) opened new perspectives in the neuroscience and neuroengineering fields. In this paper, we present the electrophysiological characterization of excitatory h-iPSCs-derived neuronal networks obtained from healthy subjects. We recorded the neural activity from 35 up to 91 days in vitro, and we extrapolated the main features describing both firing and bursting patterns. With this work, we provide a solid baseline for future exploitation of in vitro human-derived experimental models in the field of personalized medicine.
Development of excitatory neuronal networks derived from human induced pluripotent stem cells
Parodi G.;Chiappalone M.;Martinoia S.
2023-01-01
Abstract
The combination of neuronal networks derived from human induced pluripotent stem cells (h-iPSCs) with micro-electrode arrays (MEAs) opened new perspectives in the neuroscience and neuroengineering fields. In this paper, we present the electrophysiological characterization of excitatory h-iPSCs-derived neuronal networks obtained from healthy subjects. We recorded the neural activity from 35 up to 91 days in vitro, and we extrapolated the main features describing both firing and bursting patterns. With this work, we provide a solid baseline for future exploitation of in vitro human-derived experimental models in the field of personalized medicine.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
