Total Antioxidant Capacity (TAC), the complete pattern of antioxidant molecules fighting free radicals within the body, represents a fundamental biomarker for monitoring the health status of the organism [1]. To date, several assays to measure TAC are commercially available [2]. However, their application is often limited by inaccuracy, time- and cost-consuming procedures, and invasiveness [2]. To address this, we developed an innovative detection scheme exploiting the peroxidase enzyme-like properties of 4 nm platinum nanoparticles (PtNPs), combined with biologically relevant radical probes (hydroxyl radicals), for an accurate and rapid (5 minutes) assessment of the body TAC. 3,3’,5,5’-tetramethylbenzidine (TMB) is used as chromogenic probe, allowing the naked-eye evaluation of the total antioxidant content of the sample [3]. Furthermore, we developed a multi-line Pt-based lateral flow device, relying on three sequential test lines with increasing concentrations of Pt nanozymes. Saliva sample flows along the device, first encountering TMB and then the detection zone, coloring a number of blue lines that is proportional to the level of antioxidants. This non-invasive colorimetric nanosensor provides a rapid, easy, and naked-eye discrimination of the body TAC (Fig. 1). Figure 1: (A) Scheme of the multi-line Pt-based lateral flow device. (B) Representative pictures of three devices used to determine the antioxidant levels of three saliva samples.
Multi-line Pt-based lateral flow device for colorimetric measurement of antioxidant levels in saliva
Anna Scarsi;Pier Paolo Pompa
2021-01-01
Abstract
Total Antioxidant Capacity (TAC), the complete pattern of antioxidant molecules fighting free radicals within the body, represents a fundamental biomarker for monitoring the health status of the organism [1]. To date, several assays to measure TAC are commercially available [2]. However, their application is often limited by inaccuracy, time- and cost-consuming procedures, and invasiveness [2]. To address this, we developed an innovative detection scheme exploiting the peroxidase enzyme-like properties of 4 nm platinum nanoparticles (PtNPs), combined with biologically relevant radical probes (hydroxyl radicals), for an accurate and rapid (5 minutes) assessment of the body TAC. 3,3’,5,5’-tetramethylbenzidine (TMB) is used as chromogenic probe, allowing the naked-eye evaluation of the total antioxidant content of the sample [3]. Furthermore, we developed a multi-line Pt-based lateral flow device, relying on three sequential test lines with increasing concentrations of Pt nanozymes. Saliva sample flows along the device, first encountering TMB and then the detection zone, coloring a number of blue lines that is proportional to the level of antioxidants. This non-invasive colorimetric nanosensor provides a rapid, easy, and naked-eye discrimination of the body TAC (Fig. 1). Figure 1: (A) Scheme of the multi-line Pt-based lateral flow device. (B) Representative pictures of three devices used to determine the antioxidant levels of three saliva samples.
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