Signalling from astrocyte processes in CNS

The role of astrocytes in the Central Nervous System (CNS) has been increasingly recognized in the last decades. Astrocytes, indeed, not only support neurons but also play a key role in signal transmission in CNS releasing gliotransmitters as glutamate and participating to the tripartite synpases. Here, astrocytes function in signal transmission was modeled using ex-vivo models purified from adult rat brain, named gliosomes. Using the perfusion technique, release from gliosomes was tested in three different contexts: - glutamate release associated to receptor-receptor interactions, - exosomes release, - glutamate release evoked by photobiomodulation stimulation.