Objective: Precocious structural degeneration of bioprostheses in irradiated survivors of chest malignancies has been reported. Aim of this in-vitro study was to evaluate any structural and/or biomechanical radiation-induced changes on a fixed bovine peri-cardium used to manufacture prosthetic heart valve leaflets. Methods: Fixed-pericardial bovine patches underwent irradia-tion with a c-rays source IBL 437C, according to experimental radiobiological protocols (T1: single-2-Gray (Gy) dose in 15+/- sec; T2: single-10-Gy dose in 76+/-7 sec; T3: single-30-Gy dose in 230 sec; T4: fractioned radiation 3-Gy dose in 23 sec /session, 910 sessions; T0: controls). Specimen assessment included mechanical traction test (strain-stress and load to breakage point), shrinkage test (contraction temperature) and histological examination (hematoxylin/eosin for tissue features, Azan for collagen fibers, Verhoeff/VanGieson for elastin fibers). Results: Mechanical traction tests did not show remarkable variations in traction load values. Contrac-tion temperature (°C) did not show any significant difference among the specimen, with only a trend towards a mild increase in the mean shrinkage temperature in all radiated samples vs T0. There were no remarkable histological differences, with irradiated samples maintain-ing the same architecture of elastic fibers and collagen bundles, as well as the same cellular and vascular content of controls. Conclusions: In-vitro, ionizing radiations do not seem to induce any biomechanical and/or structural changes on fixed bovine pericardium. In-vivo studies are required to evaluate the potential role of endogenous factors in inducing radiation mediated prostheses tissue degeneration.

Structural and biomechanical properties of fixed bovine pericardium heart valve prostheses are not affected by ionizing radiation: an in-vitro study

G. M. Olivieri;C. Brega;A. Salsano;E. Sportelli;N. Di Lorenzo;S. Vagge;R. Corvò;F. Santini
2017

Abstract

Objective: Precocious structural degeneration of bioprostheses in irradiated survivors of chest malignancies has been reported. Aim of this in-vitro study was to evaluate any structural and/or biomechanical radiation-induced changes on a fixed bovine peri-cardium used to manufacture prosthetic heart valve leaflets. Methods: Fixed-pericardial bovine patches underwent irradia-tion with a c-rays source IBL 437C, according to experimental radiobiological protocols (T1: single-2-Gray (Gy) dose in 15+/- sec; T2: single-10-Gy dose in 76+/-7 sec; T3: single-30-Gy dose in 230 sec; T4: fractioned radiation 3-Gy dose in 23 sec /session, 910 sessions; T0: controls). Specimen assessment included mechanical traction test (strain-stress and load to breakage point), shrinkage test (contraction temperature) and histological examination (hematoxylin/eosin for tissue features, Azan for collagen fibers, Verhoeff/VanGieson for elastin fibers). Results: Mechanical traction tests did not show remarkable variations in traction load values. Contrac-tion temperature (°C) did not show any significant difference among the specimen, with only a trend towards a mild increase in the mean shrinkage temperature in all radiated samples vs T0. There were no remarkable histological differences, with irradiated samples maintain-ing the same architecture of elastic fibers and collagen bundles, as well as the same cellular and vascular content of controls. Conclusions: In-vitro, ionizing radiations do not seem to induce any biomechanical and/or structural changes on fixed bovine pericardium. In-vivo studies are required to evaluate the potential role of endogenous factors in inducing radiation mediated prostheses tissue degeneration.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11567/996872
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