Ion channels (i.c.) are transmembrane proteins involved in nearly all physiological processes and in many human diseases. Nowadays, conventional Patch-Clamp (PC) is the gold technique for both ion channel drug discovery and research. However, i.c. remain an under-exploited target class, because of the PC laborious approach. Trends in i.c. screening technologies have focused on increasing throughput of patch-clamp by planar approaches, i.e. Population PC (PPC) and Nano PC (NPC). We rely on a NPC device based on poly(dimethylsiloxane) (PDMS) planar chips because of high processability and low cost of that alternative material.
Developing poly(dimethylsiloxane) planar chips for nano patch-clamp applications
Guida P;Firpo G;Repetto L;Boragno C;Valbusa U
2010-01-01
Abstract
Ion channels (i.c.) are transmembrane proteins involved in nearly all physiological processes and in many human diseases. Nowadays, conventional Patch-Clamp (PC) is the gold technique for both ion channel drug discovery and research. However, i.c. remain an under-exploited target class, because of the PC laborious approach. Trends in i.c. screening technologies have focused on increasing throughput of patch-clamp by planar approaches, i.e. Population PC (PPC) and Nano PC (NPC). We rely on a NPC device based on poly(dimethylsiloxane) (PDMS) planar chips because of high processability and low cost of that alternative material.
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