Mediterranean Sea (covering approximately a 0.7% of the world’s ocean area) represents a significant source of marine biodiversity. Sponges represent the most studied marine organisms as resource of bioactive compounds. In fact, previous studies reported the bioactivity of many marine sponge extracts with several activities as anti-cancer, anti-inflammatory and anti-viral. In particular, some extracts have been used to produce commercial anticancer drugs. In the present study we tested the potential antiproliferative effect of two marine sponges, Oceanapia cf. perforata (Sarà, 1960) from Faro Lake (Sicily) and Agelas oroides (Schmidt, 1864) from Punta San Pancrazio (Gulf of Naples). In our previous studies these two sponges were characterized by morphological analysis and amplification of several conserved molecular markers. Here we identified two active fractions from A. oroides (both with logIC50 of 1.7) and one fraction from O. cf. perforata (logIC50 <1) able to reduce the percentage of metabolically active cells of human pancreatic cancer cell line (PaCa-2). Further bioassay-guided fractionation and chemical characterization will be performed, aiming to identify natural products from both sponges responsible of this activity.
Cell viability assay of methanolic extracts of two marine sponges on pancreatic cancer cell line
Federico S.;Bertolino M.;
2022-01-01
Abstract
Mediterranean Sea (covering approximately a 0.7% of the world’s ocean area) represents a significant source of marine biodiversity. Sponges represent the most studied marine organisms as resource of bioactive compounds. In fact, previous studies reported the bioactivity of many marine sponge extracts with several activities as anti-cancer, anti-inflammatory and anti-viral. In particular, some extracts have been used to produce commercial anticancer drugs. In the present study we tested the potential antiproliferative effect of two marine sponges, Oceanapia cf. perforata (Sarà, 1960) from Faro Lake (Sicily) and Agelas oroides (Schmidt, 1864) from Punta San Pancrazio (Gulf of Naples). In our previous studies these two sponges were characterized by morphological analysis and amplification of several conserved molecular markers. Here we identified two active fractions from A. oroides (both with logIC50 of 1.7) and one fraction from O. cf. perforata (logIC50 <1) able to reduce the percentage of metabolically active cells of human pancreatic cancer cell line (PaCa-2). Further bioassay-guided fractionation and chemical characterization will be performed, aiming to identify natural products from both sponges responsible of this activity.
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