BACKGROUND: The initial interactions between cellular components and materials play an important role in the stages of the healing process around fixture and implant abutment. These interactions are critically mediated by the state of the surface. Plasma treatment has been used for several decades to increase cellular adherence to polymeric and metallic materials. Recently it was suggested to enhance cell adherence to titanium implant surface. The present experimental study was aimed to analyze wheatear cleaning treatment using plasma of argon could interfere with cellular growth of fibroblasts on titanium turned disks at different time-points. MATERIALS & METHODS: Sixty titanium disks were dived in two groups: 30 were left untreated (control) and 30 were cleaned using plasma of argon (test). They were immersed in culture of fibroblasts (L-929) and, after preparation, stained using DIPA, staining nuclei, and fluorescent phalloidin , labeling cellular body. Count nuclei and analysis of cellular body was performed using fluorescent microscopy and imaging analysis software. Analysis was performed at different time-points: 2, 8 and 48 hours. RESULTS: At the end of this study, the control group presented fibroblast adhesion mean values of 135 (±26), 184 (±64), 372 (±67) cells/field respectively at 2,8 and 48h. The test group presented fibroblast adhesion mean values of 181 (±37), 233 (±51), 369 (±84) cells/field respectively at 2,8 and 48h. Test group data demonstrated statistical significantly higher values compared to control group at 2 (p<0.0039) and 8h (p<0.0488). Data at 48h did not presented any statistical significant difference. Analyzing cellular body, at 2 hours the cellular body is flatter and more spread , i.e. the process of cellular adherence is in a more advanced stage in the treated sample. CONCLUSIONS: Removal of organic and inorganic contamination on the surface of titanium disks using plasma of argon seems to accelerate fibroblast adhesion in the early stage (2-8h). This effect disappears in the long run due to the space saturation. According to the presented data, plasma cleaning could be applied in prosthetic treatment of implant supported rehabilitation, enhancing soft tissue response to the abutment insertion.
Cell growth on Titanium disks treated by Plasma of Argon Experimental study
Canullo L;
2011-01-01
Abstract
BACKGROUND: The initial interactions between cellular components and materials play an important role in the stages of the healing process around fixture and implant abutment. These interactions are critically mediated by the state of the surface. Plasma treatment has been used for several decades to increase cellular adherence to polymeric and metallic materials. Recently it was suggested to enhance cell adherence to titanium implant surface. The present experimental study was aimed to analyze wheatear cleaning treatment using plasma of argon could interfere with cellular growth of fibroblasts on titanium turned disks at different time-points. MATERIALS & METHODS: Sixty titanium disks were dived in two groups: 30 were left untreated (control) and 30 were cleaned using plasma of argon (test). They were immersed in culture of fibroblasts (L-929) and, after preparation, stained using DIPA, staining nuclei, and fluorescent phalloidin , labeling cellular body. Count nuclei and analysis of cellular body was performed using fluorescent microscopy and imaging analysis software. Analysis was performed at different time-points: 2, 8 and 48 hours. RESULTS: At the end of this study, the control group presented fibroblast adhesion mean values of 135 (±26), 184 (±64), 372 (±67) cells/field respectively at 2,8 and 48h. The test group presented fibroblast adhesion mean values of 181 (±37), 233 (±51), 369 (±84) cells/field respectively at 2,8 and 48h. Test group data demonstrated statistical significantly higher values compared to control group at 2 (p<0.0039) and 8h (p<0.0488). Data at 48h did not presented any statistical significant difference. Analyzing cellular body, at 2 hours the cellular body is flatter and more spread , i.e. the process of cellular adherence is in a more advanced stage in the treated sample. CONCLUSIONS: Removal of organic and inorganic contamination on the surface of titanium disks using plasma of argon seems to accelerate fibroblast adhesion in the early stage (2-8h). This effect disappears in the long run due to the space saturation. According to the presented data, plasma cleaning could be applied in prosthetic treatment of implant supported rehabilitation, enhancing soft tissue response to the abutment insertion.
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