o overcome the optical diffraction limit, several super-resolution microscopy techniques have been demonstrated. Circular intensity differential scattering (CIDS) based on polarized light scattering is a powerful super-resolution and noninvasive microscopy technique to crumble the abbe optical resolution limit for studying and monitoring biological particles. In this work, a phasor plot approach combined with CIDS microscopy has provided a molecular view of sample to recognize the presence of different molecular species in a pixel. The data in terms of polarization response at each pixel can be mapped to a single point called phasor. The phasor approach has also the potential to simplify the data analyzed in CIDS, paving the way for the analysis of large data sets and feasible to interpret for the non-expert in data analysis.

Resolution Improvement in CIDS Super-Resolution Microscopy using a Phasor Plot Approach

Mohebi, Ali;Marongiu, Riccardo;Callegari, Fabio;Diaspro, Alberto
2021

Abstract

o overcome the optical diffraction limit, several super-resolution microscopy techniques have been demonstrated. Circular intensity differential scattering (CIDS) based on polarized light scattering is a powerful super-resolution and noninvasive microscopy technique to crumble the abbe optical resolution limit for studying and monitoring biological particles. In this work, a phasor plot approach combined with CIDS microscopy has provided a molecular view of sample to recognize the presence of different molecular species in a pixel. The data in terms of polarization response at each pixel can be mapped to a single point called phasor. The phasor approach has also the potential to simplify the data analyzed in CIDS, paving the way for the analysis of large data sets and feasible to interpret for the non-expert in data analysis.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11567/1073371
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