In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy (PALM ), stochastic optical reconstruction microscopy (STORM), point accumulation for imaging in nanoscale topography (PAINT), coordinate targeted fluorescence microscopy (STED, reversible saturable optical fluorescence transition (RESOLFT), structured illumination microscopy, expansion microscopy (ExM), and liquid tunable microscopy (LIQUITOPY).
Fluorescence microscopy
Diaspro A.;Oneto M.;Pesce L.;Cainero I.
2019-01-01
Abstract
In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy (PALM ), stochastic optical reconstruction microscopy (STORM), point accumulation for imaging in nanoscale topography (PAINT), coordinate targeted fluorescence microscopy (STED, reversible saturable optical fluorescence transition (RESOLFT), structured illumination microscopy, expansion microscopy (ExM), and liquid tunable microscopy (LIQUITOPY).
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